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The objective of this study was to determine the capability of Raman spectroscopy to detect several different dye classes and colors on a variety of textile fibers. To test this, four categories of dyes, reactive, disperse, acid, and direct were examined with Raman microscopy while applied to one of five fiber types (cotton, polyester, nylon, wool, and rayon). Each dye category was tested using four colors, black, blue, red, and yellow, while at four concentrations of dye (w\/w), 4% (black only), 1%, 0.5%, and 0.05% (blue, red, and yellow). Finally, each dye, fiber, color, and dye concentration combination were examined with Raman using one of two laser excitation sources (532\u2009nm and 780\u2009nm) while mounted in one of two mounting media, Permount\u2122 and Entellan\u00ae new, as well as unmounted. Raman spectroscopy could detect some dyes at low concentrations (0.5% and 0.05%) even when mounted in mounting media and covered with a glass coverslip. Excitation source, dye category, dye concentration, fiber type, and mounting method all influence the ability to detect any given dye. These results support the continued study of Raman as a tool for the examination of fiber dyes as it has shown the potential to be effective even under constraints experienced by forensic examiners.<\/jats:p>","DOI":"10.1111\/1556-4029.15125","type":"journal-article","created":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T04:19:51Z","timestamp":1662697191000},"page":"2278-2290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of detection limits for dyed and mounted textile fibers using Raman spectroscopy"],"prefix":"10.1111","volume":"67","author":[{"given":"Patrick M.","family":"Rydzak","sequence":"first","affiliation":[{"name":"Visiting Scientist Program, Federal Bureau of Investigation Laboratory Division, Research and Support Unit  Quantico Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyleen E.","family":"Elwick","sequence":"additional","affiliation":[{"name":"Visiting Scientist Program, Federal Bureau of Investigation Laboratory Division, Research and Support Unit  Quantico Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Damaso","sequence":"additional","affiliation":[{"name":"Visiting Scientist Program, Federal Bureau of Investigation Laboratory Division, Research and Support Unit  Quantico Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Federal Bureau of Investigation Laboratory Division, Research and Support Unit  Quantico Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"xi","volume-title":"Mute witnesses: trace evidence analysis","author":"Houck MM","year":"2001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/9781119373438","volume-title":"Handbook of trace evidence analysis","author":"Desiderio VJ","year":"2020"},{"key":"e_1_2_10_4_1","first-page":"89","volume-title":"Forensic examination of fibres","author":"Robertson J","year":"1999"},{"key":"e_1_2_10_5_1","first-page":"1","volume-title":"Hairs, fibers, crime, and evidence, part 2: fiber evidence","author":"Deedrick DW","year":"2000"},{"key":"e_1_2_10_6_1","first-page":"16","volume-title":"Forensic examination of fibres","author":"David SK","year":"1999"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"71","article-title":"Seeing color: practical methods in pigment microscopy","volume":"62","author":"Palenik CS","year":"2014","journal-title":"Microscope"},{"key":"e_1_2_10_8_1","unstructured":"The colour index\u2122.Society of Dyers and Colourists and American Association of Textile Chemists and Colorists 2021[cited 4 Aug 2022]. 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