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The initial study analyzed fortified samples for blood alcohol concentration (BAC) using two sizes of gray\u2010top tubes: a 10\u2010ml tube containing a nominal 1% sodium fluoride (NaF), a preservative, and a 6\u2010ml tube containing 0.25% NaF, using the variables of time, storage temperature, fill volume, and concentration. From that study, paired <jats:italic>t<\/jats:italic>\u2010tests determined no difference in BAC from data sets of the two tube sizes, however, Analysis of Variance, or ANOVA, testing determined the two tube sizes yielded different results at 0.08\u2009g\/100\u2009ml. To investigate this potential disparity, this study fortified new samples at 0.08\u2009g\/100 and focused on two variables: fill volume and storage duration, while increasing the sample population (to <jats:italic>n<\/jats:italic>\u00a0=\u00a08). For this study, the two tube types yielded equivalent concentrations under the majority of conditions studied. Differences between the two tube types were found using paired <jats:italic>t<\/jats:italic>\u2010test for the high\u2010volume samples on Days 7 and 30; ANOVA yielded the same results but also determined one additional statistical difference for the Day 30 low\u2010volume samples. However, the differences observed between tube sizes fall within standard deviation ranges established for the analytical method precision profile, indicating statistical differences are not analytically significant. Further studies are needed to investigate the comparability of the tubes under real\u2010world conditions, under which oxygen and\/or additives are not added during sample preparation steps or by usage of blood bank products.<\/jats:p>","DOI":"10.1111\/1556-4029.15172","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T07:09:33Z","timestamp":1667977773000},"page":"301-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Statistical comparisons of 0.08\u2009g\/<scp>100\u2009ml<\/scp> fortified blood alcohol samples from <scp>6\u2010ml<\/scp> and <scp>10\u2010ml<\/scp> gray\u2010top tubes"],"prefix":"10.1111","volume":"68","author":[{"given":"Maria L.","family":"Olds","sequence":"first","affiliation":[{"name":"Fort Worth Police Department Crime Laboratory  Fort Worth Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556\u20104029.14632"},{"key":"e_1_2_7_3_1","unstructured":"Becton\u2010Dickinson (BD).Amended urgent medical device recall \u2013 BD Vacutainer\u00ae Fluoride Tubes for Blood Alcohol Determinations;2019. 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