{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:48:18Z","timestamp":1790909298337,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vortioxetine is an antidepressant recently licensed in USA and EU for the treatment of major depressive disorder. Neither fatal case due to overdose nor data about postmortem concentrations on blood or other specimens have been reported. The aims of this study were the development and validation of a method for vortioxetine analysis by Liquid Chromatography Tandem Mass Spectrometry (LC\u2013MS\/MS) in postmortem samples and its application in an authentic case. The method was validated and applied on blood, vitreous humor, bile, brain, liver, kidney, and gastric content. After protein precipitation, the supernatant was directly injected into LC\u2013MS\/MS. Analysis was carried out by Multiple Reaction Monitoring (MRM) mode. The authentic case concerned a 38\u2009years\u2010old woman, affected by depression, who was found hanged at home. The method determined an acceptable sensitivity, selectivity, linearity, precision, and accuracy for all matrices. No interference was shown for all matrices. The matrices do not significantly reduce the peak intensity of vortioxetine. No carryover was shown. Toxicological analysis of the authentic case showed vortioxetine in blood (234\u2009ng\/ml), vitreous humor (10.5\u00a0ng\/ml), brain (490\u2009ng\/g), lung (479\u2009ng\/g), liver (3751\u2009ng\/g), kidney (798\u2009ng\/g), bile (2267\u2009ng\/ml) and gastric content (253\u2009ng\/ml). Our case suggests that even at blood concentrations of vortioxetine equal to 234\u2009ng\/ml, the subject was able to stage and carry out the hanging. Vortioxetine concentrations found in the other cadaveric samples (biological fluids, organs, and gastric content) may be helpful to evaluate further similar comparable cases.<\/jats:p>","DOI":"10.1111\/1556-4029.15198","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T16:25:36Z","timestamp":1673627136000},"page":"578-585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development and validation of a <scp>LC\u2013MS<\/scp>\/<scp>MS<\/scp> method for analysis of vortioxetine in postmortem specimens. First data from an authentic case"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7425-0032","authenticated-orcid":false,"given":"Pietro","family":"Zuccarello","sequence":"first","affiliation":[{"name":"Laboratory of Forensic Toxicology, Department \u201cG.F. Ingrassia\u201d University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Carnazza","sequence":"additional","affiliation":[{"name":"Laboratory of Forensic Toxicology, Department \u201cG.F. Ingrassia\u201d University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunziata","family":"Barbera","sequence":"additional","affiliation":[{"name":"Laboratory of Forensic Toxicology, Department \u201cG.F. Ingrassia\u201d University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD011520.pub2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262\u2010017\u20100612\u20107"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0269881116628440"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.10.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm101459g"},{"issue":"1","key":"e_1_2_9_7_1","first-page":"36","article-title":"Vortioxetine (brintellix): a new serotonergic antidepressant","volume":"40","author":"D'Agostino A","year":"2015","journal-title":"Pharm Ther"},{"key":"e_1_2_9_8_1","unstructured":"European Medicines Agency.Brintellix: EPAR \u2013 Summary for the public.2018.https:\/\/www.ema.europa.eu\/en\/documents\/product\u2010information\/brintellix\u2010epar\u2010product\u2010information_en.pdf. 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