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The DNA in these samples can be highly degraded and chemically damaged, making downstream genotyping using short tandem repeats (STRs) challenging. Therefore, the use of alternative genetic markers, methods that pre\u2010amplify the low amount of good quality DNA present, or methods that repair the damaged DNA template may provide more probative genetic information. This study investigated whether whole genome amplification (WGA) and DNA repair could improve STR typing of formaldehyde\u2010damaged (FD) tissues from embalmed cadavers. Additionally, comparative genotyping success using bi\u2010allelic markers, including INDELs and SNPs, was explored. Calculated random match probabilities (RMPs) using traditional STRs, INDEL markers, and two next generation sequencing (NGS) panels were compared across all samples. Overall, results showed that neither WGA nor DNA repair substantially improved STR success rates from formalin\u2010fixed tissue samples. However, when DNA from FD samples was genotyped using INDEL and SNP\u2010based panels, the RMP of each sample was markedly lower than the RMPs calculated from partial STR profiles. Therefore, the results of this study suggest that rather than attempting to improve the quantity and quality of severely damaged and degraded DNA prior to STR typing, a more productive approach may be to target smaller amplicons to provide more discriminatory DNA identifications. Furthermore, an NGS panel with less loci may yield better results when examining FD samples, due to more optimized chemistries that result in greater allelic balance and amplicon coverage.<\/jats:p>","DOI":"10.1111\/1556-4029.15200","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:33:15Z","timestamp":1675308795000},"page":"596-607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The effectiveness of various strategies to improve <scp>DNA<\/scp> analysis of formaldehyde\u2010damaged tissues from embalmed cadavers for human identification purposes"],"prefix":"10.1111","volume":"68","author":[{"given":"Natalia","family":"Czado","sequence":"first","affiliation":[{"name":"Department of Forensic Science, College of Criminal Justice Sam Houston State University  Huntsville Texas 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