{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:38:14Z","timestamp":1788349094169,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zopiclone (ZOP) is a hypnotic drug prescribed to treat insomnia. Due to the chiral nature of ZOP, the psychologically active <jats:italic>S<\/jats:italic>\u2010form and inactive <jats:italic>R<\/jats:italic>\u2010form need to be determined enantiomerically in a forensic drug analysis. In the present study, a supercritical fluid chromatography (SFC) method was designed with a faster analysis ability than that of previously reported techniques. The SFC\u2013tandem mass spectrometry (SFC\u2013MS\/MS) method was optimized using a column with a chiral polysaccharide stationary phase (Trefoil CEL2). ZOP was extracted from pooled human serum using solid\u2010phase extraction (Oasis HLB) and analyzed. The developed SFC\u2013MS\/MS method achieved the baseline separation of <jats:italic>S<\/jats:italic>\u2010ZOP and <jats:italic>R<\/jats:italic>\u2010ZOP within 2\u2009min. The fit\u2010for\u2010purpose method validation indicated that the optimized solid\u2010phase extraction achieved near complete recovery and approximately 70% of the matrix effect. Both the retention time and peak area showed sufficient precision. The lower and upper limits of quantification (LOQ) were 5.7\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>\u2009ng\/mL and 25\u2009ng\/mL for <jats:italic>R<\/jats:italic>\u2010ZOP, and 5.2\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>\u2009ng\/mL and 25\u2009ng\/mL for <jats:italic>S<\/jats:italic>\u2010ZOP. The calibration line was linear in the range from lower LOQ to upper LOQ. The stability test indicated that ZOP in serum stored in a refrigerator (4\u00b0C) degraded and about 55% remained in 31\u2009days. The quick analysis of the SFC\u2013MS\/MS method makes it a valid option for the enantiomeric analysis of ZOP.<\/jats:p>","DOI":"10.1111\/1556-4029.15279","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T06:39:46Z","timestamp":1684391986000},"page":"1352-1358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7626-3331","authenticated-orcid":false,"given":"Hiroki","family":"Segawa","sequence":"first","affiliation":[{"name":"National Research Institute of Police Science  Kashiwa Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko T.","family":"Iwata","sequence":"additional","affiliation":[{"name":"National Research Institute of Police Science  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