{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:05:30Z","timestamp":1792065930208,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A variety of LSD analogs have emerged in recent years with dual purposes of avoiding prosecution from possession while providing new options for those willing to experiment with hallucinogenic drugs. In this study, a previously published automated sample preparation method for LSD and its primary metabolite (OH\u2010LSD) was utilized to extract LSD, OH\u2010LSD, and nine LSD analogs from urine. The liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS) method was modified from the previously published LC conditions to utilize a different analytical column and gradient elution program. Mobile phases of 10\u2009mM ammonium formate with 0.1% formic acid in deionized water (mobile phase A) and 0.1% formic acid in methanol (mobile phase B) were employed. The method was validated to ANSI\/ASB Standard 036 with a 0.1\u2009ng\/mL limit of detection for all analytes and was utilized for the analysis of 325 urine specimens. Although no LSD analogs were observed in the samples analyzed, this validated method was demonstrated to be suitable for the analysis of these compounds in laboratories seeking to expand their testing scope. Automated sample preparation allows for the efficient analysis of these analytically challenging compounds with minimal manual handling. Additionally, there was no increased analytical time burden when the LC column and gradient were modified to target nine additional analytes. Detection may improve as new reference standards are developed to allow laboratories to focus on the metabolic products of these analogs. For now, this validated procedure can assist with the routine analysis and surveillance of these emerging substances.<\/jats:p>","DOI":"10.1111\/1556-4029.15572","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:39:51Z","timestamp":1719535191000},"page":"1789-1798","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Modification of a liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) method targeting lysergic acid diethylamide (\n                    <scp>LSD<\/scp>\n                    ) and its primary metabolite (\n                    <scp>OH<\/scp>\n                    \u2010\n                    <scp>LSD<\/scp>\n                    ) to include nine\n                    <scp>LSD<\/scp>\n                    analogs"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9103-8770","authenticated-orcid":false,"given":"Amy L.","family":"Patton","sequence":"first","affiliation":[{"name":"Division of Forensic Toxicology Armed Forces Medical Examiner System  Dover Delaware USA"},{"name":"SNA International LLC, Contractor Supporting the Armed Forces Medical Examiner System  Alexandria Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin L.","family":"Karschner","sequence":"additional","affiliation":[{"name":"Division of Forensic Toxicology Armed Forces Medical Examiner System  Dover Delaware USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey P.","family":"Walterscheid","sequence":"additional","affiliation":[{"name":"Division of Forensic Toxicology Armed Forces Medical Examiner System  Dover Delaware USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Division of Forensic Toxicology Armed Forces Medical Examiner System  Dover Delaware USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.8b00043"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755\u20105949.2008.00059.x"},{"key":"e_1_2_10_4_1","unstructured":"Drug Enforcement Agency.LSD drug fact sheet. [cited 2024 Feb 25]. Available from:https:\/\/www.dea.gov\/sites\/default\/files\/2020\u201006\/LSD\u20102020_0.pdf"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104684\u20108306\u20102_17"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17111953"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/13638490601106277"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.15288\/qjsa.1964.25.333"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213\u2010017\u20104777\u20104"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112203"},{"key":"e_1_2_10_11_1","unstructured":"NuwerR.Americans increase LSD use\u2014and a bleak outlook for the world may be to blame. [cited 2024 Feb 25]. Available from:https:\/\/www.scientificamerican.com\/article\/americans\u2010increase\u2010lsd\u2010use\u2010and\u2010a\u2010bleak\u2010outlook\u2010for\u2010the\u2010world\u2010may\u2010be\u2010to\u2010blame1\/"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugpo.2016.10.020"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugpo.2016.11.007"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1208\/aapsj070235"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033291715002901"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2014.11.015"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00147a022"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/24.7.550"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/03602532.2019.1638931"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1985"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556\u20104029.15224"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1884"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010018\u20101558\u20109"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2019.107856"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2613"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2789"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419\u2010023\u201000661\u20101"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkad012"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337\u2010004\u20100344\u20103"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/21.6.459"},{"key":"e_1_2_10_31_1","volume-title":"ANSI\/ASB standard 036: standard practices for method validation in forensic toxicology","author":"AAFS Standards Board","year":"2019"},{"key":"e_1_2_10_32_1","volume-title":"DoD instruction 1010.16: technical procedures for the military drug abuse testing program","author":"Office of the Under Secretary of Defense for Personnel and Readiness","year":"2020"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/12.1.1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/22.6.520"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3281"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2016.08.013"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/hup.2599"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bku084"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4142527"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104347(98)00574\u2010X"}],"container-title":["Journal of Forensic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1556-4029.15572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,29]],"date-time":"2024-12-29T23:47:24Z","timestamp":1735516044000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1556-4029.15572"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,27]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/1556-4029.15572"],"URL":"https:\/\/doi.org\/10.1111\/1556-4029.15572","archive":["Portico"],"relation":{},"ISSN":["0022-1198","1556-4029"],"issn-type":[{"value":"0022-1198","type":"print"},{"value":"1556-4029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,27]]},"assertion":[{"value":"2024-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}