{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:26:27Z","timestamp":1747182387778,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The larval excretions\/secretions (ES) of blowflies contain proteolytic enzymes and bacteria that assist with tissue breakdown. Decomposition fluid (DF) contains organic and inorganic waste products from cell death. This study investigated if human DNA recovery from blood was impacted by exposure to ES and DF over time. <jats:italic>Lucilia sericata<\/jats:italic> ES were collected daily from 50 larvae, and all available DF was collected from two fetal piglets left to decompose for 2\u2009weeks. Daily for 3\u20135\u2009days, 28\u2009\u03bcL\u201030\u2009\u03bcL of ES, DF, or a 1:1 mixture of the fluids was added to 30\u2009\u03bcL of blood on cotton. Three bloodstains per treatment were sampled every 12\u2009h up to 3\u2009days and at 1 and 2\u2009weeks after initial addition of fluid. No PCR inhibition was detected, but DNA degradation increased over time, primarily in samples exposed to ES and ES\/DF mixtures. The amount of DNA recovered decreased over time, but generally more DNA was recovered from DF samples than other samples. Full profiles, or partial profiles suitable for routine database searching (14\u201339 alleles), were generated from all DF and ES samples and at least one mixture sample at all timepoints. Partial profiles of between 1 and 13 alleles were obtained from all other mixture samples, except one mixture sample which generated no profile. These findings indicate bloodstain evidence recovered from maggot\u2010infested and\/or decomposing bodies may generate forensically useful DNA evidence and should be analyzed as quickly as possible after collection or stored appropriately to prevent further degradation.<\/jats:p>","DOI":"10.1111\/1556-4029.15597","type":"journal-article","created":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T06:44:15Z","timestamp":1726901055000},"page":"2082-2090","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The effect of insect excretions\/secretions and decomposition fluid on <scp>DNA<\/scp> quantity and quality in human bloodstains"],"prefix":"10.1111","volume":"69","author":[{"given":"Maggie","family":"Murphy","sequence":"first","affiliation":[{"name":"School of Life and Environmental Sciences Deakin University  Waurn Ponds Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Harvey","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences Deakin University  Waurn Ponds Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland A. 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