{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:43:28Z","timestamp":1791434608491,"version":"4.3.3"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Determination of alcohol markers in post\u2010mortem cases can be useful to classify drinking habits and potential alcohol habituation prior to death. Phosphatidylethanol (PEth) is a direct alcohol marker and is already commonly used in a variety of contexts. However, its use in the field of post\u2010mortem toxicology has been scarcely investigated so far. To evaluate its validity, PEth was determined in routinely collected post\u2010mortem heart blood and femoral vein blood. The stability of PEth under routine storage conditions (\u221220\u00b0C) for a period of 60\u2009days was examined. Post\u2010mortem blood was collected during medicolegal autopsies and aliquoted. Parts of the samples were used to create dried blood spots (DBS) directly after collection. Further DBS were created using aliquots stored at \u221220\u00b0C on days 1, 2, 7, 14, 30, and 60. LC\u2013MS\/MS was used for quantitative PEth analysis, and initial blood alcohol was determined using GC\u2010FID. Blood was collected from 50 different post\u2010mortem cases. The heart blood\/femoral blood ratio of PEth concentrations varied from 0.32 to 2.36 (mean\u2009=\u2009approx. 1.00), indicating a good comparability in total but a strong interindividual variation. In all PEth\u2010positive samples, the PEth concentrations increased by approximately 20% after 24\u2009hours and 70% after 60\u2009days. Post\u2010sampling formation of PEth was also found in blood samples without detectable amounts of ethanol. Neither storage at \u221280\u00b0C nor the addition of sodium metavanadate led to satisfactory stability of PEth. Based on our findings, the (sole) use of PEth for post\u2010mortem toxicology caseworks is not recommended.<\/jats:p>","DOI":"10.1111\/1556-4029.70106","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T23:59:33Z","timestamp":1750982373000},"page":"1963-1972","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phosphatidylethanol in post\u2010mortem blood: A comparative study of blood matrices and its stability at routine storage conditions"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4085-2219","authenticated-orcid":false,"given":"Jeremai","family":"Hose","sequence":"first","affiliation":[{"name":"Institute of Legal Medicine Faculty of Medicine and University Hospital, University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8779-8902","authenticated-orcid":false,"given":"Hilke","family":"Andresen\u2010Streichert","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine Faculty of Medicine and University Hospital, University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[{"name":"Centre for Preventive Doping Research\/Institute of Biochemistry German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus A.","family":"Rothschild","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine Faculty of Medicine and University Hospital, University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Juebner","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine Faculty of Medicine and University Hospital, University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.23907\/2014.022"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/wfs2.1353"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25122724"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2010.02.021"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000433\u2010199603000\u201000002"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2006.02.045"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010020\u201002415\u20109"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010008\u20100245\u20103"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2014.05.022"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/wfs2.1456"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010005\u20105195\u2010z"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022422418388"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u2010291x(87)91507\u20105"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.1987.tb05707.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010010\u20103458\u20105"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.09.068"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241512175"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3559"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkaa082"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010012\u20100799\u2010y"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinms.2018.12.002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agv120"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010015\u20109169\u20101"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004140100206"},{"key":"e_1_2_11_26_1","unstructured":"Deutsche Gesellschaft f\u00fcr Rechtsmedizin [German Society for Legal Medicine].Die rechtsmedizinische Leichen\u00f6ffnung. 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