{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T19:03:13Z","timestamp":1762282993880,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T00:00:00Z","timestamp":1751760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Forensic entomology has become an essential tool for estimating the minimum postmortem interval (mPMI), especially in scenarios where traditional methods are limited. This study aimed to characterize and differentiate the chemical profiles of three major forensically relevant fly species\u2014\n                    <jats:italic>Chrysomya albiceps<\/jats:italic>\n                    ,\n                    <jats:italic>Cochliomyia macellaria<\/jats:italic>\n                    , and\n                    <jats:italic>Lucilia cuprina<\/jats:italic>\n                    \u2014across their developmental stages. Changes in chemical profiles during larval, pupal, and pharate adult stages were monitored via ethanol\u2010based solvent extraction followed by direct infusion mass spectrometry (DIMS). Distinct chemical fingerprints were observed for each stage, with specific variations in ion compositions reflecting the dynamic nature of insect development. Chemometric analysis using PLS\u2010DA models clearly distinguished the species and their developmental stages, especially during the larval phase, which is critical for mPMI estimation. These findings demonstrate that metabolomic profiling combined with chemometric tools offers, in forensic investigations, a precise and robust approach to estimate mPMI and to identify fly species in their larval stages.\n                  <\/jats:p>","DOI":"10.1111\/1556-4029.70126","type":"journal-article","created":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T23:50:09Z","timestamp":1751845809000},"page":"2381-2392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical profiling of developmental stages in three major flies via direct infusion mass spectrometry: Improving estimates of minimum postmortem intervals in forensic entomology"],"prefix":"10.1111","volume":"70","author":[{"given":"Davi Rodrigues","family":"da Silva","sequence":"first","affiliation":[{"name":"Graduate Program in Materials Engineering and Nanotechnology (PPGEMN) Mackenzie Presbyterian University  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"MackGraphe\u2014Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian Institute  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Forensic Science Superintendency of the State of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia Camacho","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Graduate Program in Materials Engineering and Nanotechnology (PPGEMN) Mackenzie Presbyterian University  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iasmim Lopes","family":"de Lima","sequence":"additional","affiliation":[{"name":"Graduate Program in Materials Engineering and Nanotechnology (PPGEMN) Mackenzie Presbyterian University  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"MackGraphe\u2014Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian Institute  S\u00e3o Paulo 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Magalh\u00e3es","family":"Borges","sequence":"additional","affiliation":[{"name":"Graduate Program in Materials Engineering and Nanotechnology (PPGEMN) Mackenzie Presbyterian University  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"MackGraphe\u2014Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian Institute  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alane Beatriz","family":"Vermelho","sequence":"additional","affiliation":[{"name":"BIOINOVAR\u2010Biotechnology Laboratories, Biocatalysis, Bioproducts and Bioenergy, Institute of Microbiology Paulo de G\u00f3es Federal University of Rio de Janeiro (UFRJ)  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9417-8114","authenticated-orcid":false,"given":"Jez Willian Batista","family":"Braga","sequence":"additional","affiliation":[{"name":"Institute of 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