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This study aimed to determine insect colonization and succession patterns on pig carcasses inoculated with semen and to assess the feasibility of recovering Y\u2010chromosomal short tandem repeat (Y\u2010STR) DNA from fly larvae, fly pupae, and beetles. Three female pigs were used as models to simulate sexual homicide scenarios, and an indoor control experiment using chicken livers mixed with 3\u2009mL of semen was also conducted. Larvae and pupae were collected throughout the decomposition process, with crop\/gut contents dissected for DNA extraction using the phenol\u2013chloroform method. DNA concentrations were quantified by qPCR, and Y\u2010STR profiling was attempted using the Yfiler\u2122 Plus kit. In the field experiment, colonization by species from the families Calliphoridae, Sarcophagidae, Muscidae, Silphidae, Dermestidae, and Cleridae followed typical succession patterns, although arrival times varied between carcasses, with higher activity observed on semen\u2010inoculated pigs. Larvae from the indoor experiment yielded quantifiable male DNA and produced complete Y\u2010STR profiles at early time points (24\u201348\u2009h), with partial profiles thereafter (72\u201396\u2009h). In contrast, larvae from the field experiment contained only 1\u20133\u2009pg. of male DNA, insufficient for profiling. These findings demonstrate that insect crop contents can preserve male DNA and occasionally yield usable Y\u2010STR data; however, DNA quantities are often too low for successful profiling. Future studies should therefore incorporate additional genetic markers alongside conventional Y\u2010STRs to enable profiling from degraded DNA.<\/jats:p>","DOI":"10.1111\/1556-4029.70341","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T02:41:38Z","timestamp":1777084898000},"page":"1576-1591","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recovery of male\n                    <scp>DNA<\/scp>\n                    acquired from carrion\u2010feeding insects in a simulated sexual assault scenario"],"prefix":"10.1111","volume":"71","author":[{"given":"Tinotenda Angel","family":"Mupfumi","sequence":"first","affiliation":[{"name":"School of Agriculture and Science, College of Agriculture, Engineering and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenu","family":"Ghai","sequence":"additional","affiliation":[{"name":"School of Agriculture and Science, College of Agriculture, Engineering and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2625-2954","authenticated-orcid":false,"given":"Danisile","family":"Tembe","sequence":"additional","affiliation":[{"name":"School of Agriculture and Science, College of Agriculture, Engineering and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr18189"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1948550609355486"},{"key":"e_1_2_11_4_1","volume-title":"Why is crime in South Africa so violent? 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