{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:59:42Z","timestamp":1790405982909,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The identification of human remains in mass\u2010casualty incidents requires rapid and reliable forensic DNA analysis, particularly when dealing with degraded skeletal material. Although rapid forensic identification is a fundamental objective in all disaster victim identification (DVI) operations, the repatriation of deceased hostages from the October 7, 2023, attack, c. 2\u2009years postmortem (PM), imposed an additional challenge of a fixed operational deadline dictated by the implementation of the hostages\u2013prisoners exchange agreement. To accelerate STR profiling, we reconfigured the conventional sequential process (extraction, quantification, amplification) into a flexible, decision\u2010driven workflow. The new workflow incorporates a shortened 1.5\u2010h DNA incubation, predefined dual\u2010volume PCR amplification, and deferred DNA quantification. Built\u2010in contingency mechanisms including multi\u2010sample per case and enhanced 33\u2010Cycle PCR\u2010enabled immediate progression to alternative pathways when required. The accelerated workflow was applied in a real\u2010world operational setting involving 37 cases (79 samples) collected during multiple repatriation phases. Informative STR profiles were obtained from 90% (71\/79) of samples within 4\u2009h per repatriation phase. Although eight individual samples remained non\u2010informative, the integrated contingency mechanisms enabled successful resolution of all 37 cases (100%). Informative profiles were generated across a broad range of DNA quantities, including low\u2010template samples, and from diverse skeletal elements. By comparison, the RapidHIT\u2122 system generated an informative profile in only one of 21 samples. These findings demonstrate that restructuring the DNA workflow into a flexible, decision\u2010driven process with built\u2010in contingency mechanisms enables rapid and reliable profiling. The proposed workflow provides a practical operational framework for time\u2010critical forensic DNA identification.<\/jats:p>","DOI":"10.1111\/1556-4029.70477","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:33:02Z","timestamp":1790404382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Accelerated\n                    <scp>DNA<\/scp>\n                    profiling through workflow reconfiguration: Practical experience from 2\u2010year postmortem skeletal remains in hostage repatriation"],"prefix":"10.1111","author":[{"given":"Michal Nachman","family":"Shenfeld","sequence":"first","affiliation":[{"name":"Forensic Biology Division Israel National Center of Forensic Medicine  Tel\u2010Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taly","family":"Spivak\u2010Kroizman","sequence":"additional","affiliation":[{"name":"Forensic Biology Division 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Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5719-0632","authenticated-orcid":false,"given":"Nurit","family":"Bublil","sequence":"additional","affiliation":[{"name":"Forensic Biology Division Israel National Center of Forensic Medicine  Tel\u2010Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Disaster victim identification guide","author":"INTERPOL","year":"2023"},{"key":"e_1_2_9_3_1","first-page":"1","volume-title":"Lessons learned from 9\/11: DNA identifications in mass fatality incidents","author":"Bailey\u2010Wilson 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