{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:31:56Z","timestamp":1789979516018,"version":"4.0.1"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1.5.013.12N"],"award-info":[{"award-number":["1.5.013.12N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Obtaining a better understanding of mechanisms involved in bacterial infections is of paramount importance for the development of novel agents to control disease caused by (antibiotic resistant) pathogens in aquaculture. In this study, we investigated the impact of catecholamine stress hormones on growth and virulence factor production of pathogenic vibrios (i.e. two Vibrio campbellii strains and two Vibrio anguillarum strains). Both norepinephrine and dopamine (at 100 \u03bcM) significantly induced growth in media containing serum. The compounds also increased swimming motility of the tested strains, whereas they had no effect on caseinase, chitinase, and hemolysin activities. Further, antagonists for eukaryotic catecholamine receptors were able to neutralize some of the effects of the catecholamines. Indeed, the dopaminergic receptor antagonist chlorpromazine neutralized the effect of dopamine, and the \u03b1-adrenergic receptor antagonists phentolamine and phenoxybenzamine neutralized the effect of norepinephrine, whereas the \u03b2-adrenergic receptor antagonist propranolol had limited to no effect. Finally, pretreatment of pathogenic V. campbellii with catecholamines significantly increased its virulence toward giant freshwater prawn larvae. However, the impact of catecholamine receptor antagonists on in vivo virulence was less clear-cut when compared to the in vitro experiments. In summary, our results show that \u2013 similar to enteric pathogens \u2013 catecholamines also increase the virulence of vibrios that are pathogenic to aquatic organisms by increasing motility and growth in media containing serum.<\/jats:p>","DOI":"10.1111\/1574-6941.12432","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T08:56:19Z","timestamp":1411980979000},"page":"761-769","source":"Crossref","is-referenced-by-count":25,"title":["The catecholamine stress hormones norepinephrine and dopamine increase the virulence of pathogenic <i>Vibrio anguillarum<\/i> and <i>Vibrio campbellii<\/i>"],"prefix":"10.1093","volume":"90","author":[{"given":"Gde Sasmita J.","family":"Pande","sequence":"first","affiliation":[{"name":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium"},{"name":"Department of Aquatic Resources Management, Faculty of Marine Science and Fisheries, Udayana University, Bali, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thao","family":"Suong","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bossier","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Defoirdt","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,12,1]]},"reference":[{"key":"2021103112531361200_fem12432-bib-0001","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/j.1472-765X.2006.01989.x","article-title":"Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates","volume":"43","author":"Austin","year":"2006","journal-title":"Lett Appl Microbiol"},{"key":"2021103112531361200_fem12432-bib-0002","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.aquaculture.2008.11.034","article-title":"In vivo effects of single or combined N-acyl homoserine lactone quorum sensing signals on the performance of Macrobrachium rosenbergii larvae","volume":"288","author":"Baruah","year":"2009","journal-title":"Aquaculture"},{"key":"2021103112531361200_fem12432-bib-0003","doi-asserted-by":"crossref","first-page":"4043","DOI":"10.1128\/jb.179.12.4043-4045.1997","article-title":"Cross-species induction of luminescence in the quorum sensing bacterium Vibrio harveyi","volume":"179","author":"Bassler","year":"1997","journal-title":"J Bacteriol"},{"key":"2021103112531361200_fem12432-bib-0004","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.micpath.2007.10.001","article-title":"The role of the QseC quorum-sensing sensor kinase in colonization and norepinephrine-enhanced motility of Salmonella enteric serovar Typhimurium","volume":"44","author":"Bearson","year":"2008","journal-title":"Microb Pathog"},{"key":"2021103112531361200_fem12432-bib-0005","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1098\/rstb.2010.0170","article-title":"Aquaculture: global status and trends","volume":"365","author":"Bostock","year":"2010","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"2021103112531361200_fem12432-bib-0006","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/j.1749-7345.2001.tb00922.x","article-title":"Reproductive performance of Macrobrachium rosenbergii female in captivity","volume":"32","author":"Cavalli","year":"2001","journal-title":"J World Aquaculture Soc"},{"key":"2021103112531361200_fem12432-bib-0007","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0044-8486(03)00324-7","article-title":"Physiological involvement of DA in ovarian development of the freshwater giant prawn, Macrobrachium rosenbergii","volume":"228","author":"Chen","year":"2003","journal-title":"Aquaculture"},{"key":"2021103112531361200_fem12432-bib-0008","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1136\/gut.2006.114926","article-title":"Norepinephrine increases the pathogenic potential of Campylobacter jejuni","volume":"56","author":"Cogan","year":"2007","journal-title":"Gut"},{"key":"2021103112531361200_fem12432-bib-0009","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1128\/JB.184.6.1617-1629.2002","article-title":"VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum","volume":"184","author":"Croxatto","year":"2002","journal-title":"J Bacteriol"},{"key":"2021103112531361200_fem12432-bib-0010","doi-asserted-by":"crossref","first-page":"e1003919","DOI":"10.1371\/journal.ppat.1003919","article-title":"Early mortality syndrome outbreaks: a microbial management issue in shrimp farming?","volume":"10","author":"De Schryver","year":"2014","journal-title":"PLoS Pathog"},{"key":"2021103112531361200_fem12432-bib-0011","doi-asserted-by":"crossref","first-page":"e1003603","DOI":"10.1371\/journal.ppat.1003603","article-title":"Antivirulence therapy for animal production: filling an arsenal with novel weapons for sustainable diseases control","volume":"9","author":"Defoirdt","year":"2013","journal-title":"PLoS Pathog"},{"key":"2021103112531361200_fem12432-bib-0012","doi-asserted-by":"crossref","first-page":"6419","DOI":"10.1128\/AEM.00753-06","article-title":"Quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp Artemia franciscana from pathogenic Vibrio harveyi, Vibrio campbellii and Vibrio parahaemolyticus isolates","volume":"72","author":"Defoirdt","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"2021103112531361200_fem12432-bib-0013","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.mib.2011.03.004","article-title":"Alternatives to antibiotics for control of bacterial disease in aquaculture","volume":"14","author":"Defoirdt","year":"2011","journal-title":"Curr Opin Microbiol"},{"key":"2021103112531361200_fem12432-bib-0014","volume-title":"The State of World Fisheries and Aquaculture","author":"FAO","year":"2012"},{"key":"2021103112531361200_fem12432-bib-0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/361073","article-title":"Communication between bacteria and their hosts","volume":"2013","author":"Freestone","year":"2013","journal-title":"Scientifica"},{"key":"2021103112531361200_fem12432-bib-0016","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1574-6968.1999.tb13449.x","article-title":"Stimulation of bacterial growth by heat-stable, norepinephrine-induced autoinducers","volume":"172","author":"Freestone","year":"1999","journal-title":"FEMS Microbiol Lett"},{"key":"2021103112531361200_fem12432-bib-0017","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0378-1097(03)00243-X","article-title":"Involvement of enterobactin in norepinephrine-mediated iron supply from transferrin to enterohaemorrhagic Escherichia coli","volume":"222","author":"Freestone","year":"2003","journal-title":"FEMS Microbiol Lett"},{"key":"2021103112531361200_fem12432-bib-0018","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1471-2180-7-8","article-title":"Blockade of catecholamine-induced growth by adrenergic and dopaminergic receptor antagonists in Escherichia coli O157:H7, Salmonella enterica and Yersinia enterocolitica","volume":"7","author":"Freestone","year":"2007","journal-title":"BMC Microbiol"},{"key":"2021103112531361200_fem12432-bib-0019","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.tim.2007.11.005","article-title":"Microbial endrocrinology: how stress influences susceptibility to infection","volume":"16","author":"Freestone","year":"2008","journal-title":"Trends Microbiol"},{"key":"2021103112531361200_fem12432-bib-0020","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.cbpa.2005.12.009","article-title":"Involvement of norepinephrine in the hyperglycemic response of the freshwater giant prawn, Macrobrachium rosenbergii, under cold shock","volume":"143","author":"Hsieh","year":"2006","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"2021103112531361200_fem12432-bib-0021","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.tig.2004.05.007","article-title":"Evolution of cell-cell signaling in animals: did late horizontal gene transfer from bacteria have a role?","volume":"20","author":"Iyer","year":"2004","journal-title":"Trends Genet"},{"key":"2021103112531361200_fem12432-bib-0022","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0044-8486(94)90309-3","article-title":"Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection","volume":"128","author":"Karunasagar","year":"1994","journal-title":"Aquaculture"},{"key":"2021103112531361200_fem12432-bib-0023","doi-asserted-by":"crossref","first-page":"4875","DOI":"10.1128\/IAI.00550-07","article-title":"Global effects of the cell-to-cell signaling molecules autoinducer-2, autoinducer-3, and epinephrine in luxS mutant of enterohemorragic Escherichia coli","volume":"75","author":"Kendall","year":"2007","journal-title":"Infect Immun"},{"key":"2021103112531361200_fem12432-bib-0024","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.fsi.2005.01.003","article-title":"Dopamine depresses the immune ability and increases susceptibility to Lactococcus garvieae in the freshwater giant prawn, Macrobrachium rosenbergii","volume":"19","author":"Li","year":"2005","journal-title":"Fish Shellfish Immunol"},{"key":"2021103112531361200_fem12432-bib-0025","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.tim.2003.11.004","article-title":"Microbial endocrinology and infectious disease in the 21st century","volume":"12","author":"Lyte","year":"2004","journal-title":"Trends Microbiol"},{"key":"2021103112531361200_fem12432-bib-0026","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1574-6968.1996.tb08196.x","article-title":"Production of an autoinducer of growth by norepinephrine cultured Escherichia coli O157:H7","volume":"139","author":"Lyte","year":"1996","journal-title":"FEMS Microbiol Lett"},{"key":"2021103112531361200_fem12432-bib-0027","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1111\/j.1749-7345.1976.tb00096.x","article-title":"The effects of algal supplements on static system culture on Macrobrachium rosenbergii (de Man) larvae","volume":"7","author":"Maddox","year":"1976","journal-title":"Proc World Mariculture Soc"},{"key":"2021103112531361200_fem12432-bib-0028","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1159\/000077866","article-title":"Dual flagellar systems enable motility under different circumstances","volume":"7","author":"McCarter","year":"2004","journal-title":"J Mol Microbiol Biotechnol"},{"key":"2021103112531361200_fem12432-bib-0029","article-title":"Disease control in shrimp aquaculture with probiotic bacteria","volume-title":"Proceedings of the 8th International Symposium on Microbial Ecology","author":"Moriarty","year":"1999"},{"key":"2021103112531361200_fem12432-bib-0030","first-page":"1","article-title":"Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger","volume":"52","author":"Nagpure","year":"2012","journal-title":"J Basic Microbiol"},{"key":"2021103112531361200_fem12432-bib-0031","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1472-765X.2007.02136.x","article-title":"Catecholamine-induced stimulation of growth in Vibrio species","volume":"44","author":"Nakano","year":"2007","journal-title":"Lett Appl Microbiol"},{"key":"2021103112531361200_fem12432-bib-0032","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1086\/513275","article-title":"Modulation of pathogenicity with norepinephrine related to the type III secretion system of Vibrio parahaemolyticus","volume":"195","author":"Nakano","year":"2007","journal-title":"J Infect Dis"},{"key":"2021103112531361200_fem12432-bib-0033","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.vetmic.2011.06.024","article-title":"Regulation of virulence factors by quorum sensing in Vibrio harveyi","volume":"154","author":"Natrah","year":"2011","journal-title":"Vet Microbiol"},{"key":"2021103112531361200_fem12432-bib-0034","volume-title":"Farming freshwater prawns: a manual for the culture of the giant river prawn, Macrobrachium rosenbergii","author":"New","year":"2003"},{"key":"2021103112531361200_fem12432-bib-0035","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1046\/j.1365-2958.1996.412927.x","article-title":"Chemotactic motility is required for invasion of the host by the fish pathogen Vibrio anguillarum","volume":"19","author":"O'Toole","year":"1996","journal-title":"Mol Microbiol"},{"key":"2021103112531361200_fem12432-bib-0036","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/0301-0082(95)00049-6","article-title":"The neuroimmunology of stress from invertebrates to man","volume":"48","author":"Ottaviani","year":"1996","journal-title":"Prog Neurobiol"},{"key":"2021103112531361200_fem12432-bib-0037","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s11802-014-2013-y","article-title":"Effect of salinity on the biosynthesis of amines in Litopenaeus vannamei and the expression of gill related ion transport genes","volume":"13","author":"Pan","year":"2014","journal-title":"J Ocean Univ China"},{"key":"2021103112531361200_fem12432-bib-0038","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.vetmic.2013.08.021","article-title":"The Vibrio campbellii quorum sensing signals have different impact on virulence of the bacterium towards different crustacean hosts","volume":"167","author":"Pande","year":"2013","journal-title":"Vet Microbiol"},{"key":"2021103112531361200_fem12432-bib-0039","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/j.1758-2229.2011.00268.x","article-title":"Quorum sensing regulation of virulence gene expression in Vibrio harveyi in vitro and in vivo during infection of brine shrimp (Artemia fransiscana)","volume":"3","author":"Ruwandeepika","year":"2011","journal-title":"Environ Microbiol Rep"},{"key":"2021103112531361200_fem12432-bib-0040","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0044-8486(03)00446-0","article-title":"Efficacy of orally administered florfenicol and oxolinic acid for the treatment of vibriosis in cod (Gadus morhua)","volume":"235","author":"Samuelsen","year":"2004","journal-title":"Aquaculture"},{"key":"2021103112531361200_fem12432-bib-0041","first-page":"685","article-title":"Microbial endocrinology","volume":"6","author":"Sharaff","year":"2011","journal-title":"Cent Eur J Biol"},{"key":"2021103112531361200_fem12432-bib-0042","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3354\/dao053231","article-title":"Virulence of luminous vibrios to Artemia franciscana nauplii","volume":"53","author":"Soto-Rodriguez","year":"2003","journal-title":"Dis Aquat Organ"},{"key":"2021103112531361200_fem12432-bib-0043","doi-asserted-by":"crossref","first-page":"8951","DOI":"10.1073\/pnas.1537100100","article-title":"Bacteria-host communication: the language of hormones","volume":"100","author":"Sperandio","year":"2003","journal-title":"P Natl Acad Sci USA"},{"key":"2021103112531361200_fem12432-bib-0044","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.vetmic.2011.09.012","article-title":"The complex interplay between stress and bacterial infections in animals","volume":"155","author":"Verbrugghe","year":"2012","journal-title":"Vet Microbiol"},{"key":"2021103112531361200_fem12432-bib-0045","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.fsi.2011.01.019","article-title":"QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda","volume":"30","author":"Wang","year":"2011","journal-title":"Fish Shellfish Immunol"},{"key":"2021103112531361200_fem12432-bib-0046","article-title":"Quorum Sensing positively regulates flagellar motility in pathogenic Vibrio harveyi","author":"Yang","year":"2014","journal-title":"Environ Microbiol"}],"container-title":["FEMS Microbiology Ecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsec\/article-pdf\/90\/3\/761\/40972622\/femsec_90_3_761.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsec\/article-pdf\/90\/3\/761\/40972622\/femsec_90_3_761.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T12:53:46Z","timestamp":1635684826000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsec\/article\/90\/3\/761\/540774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,12,1]]}},"URL":"https:\/\/doi.org\/10.1111\/1574-6941.12432","relation":{},"ISSN":["1574-6941","0168-6496"],"issn-type":[{"value":"1574-6941","type":"electronic"},{"value":"0168-6496","type":"print"}],"subject":[],"published-other":{"date-parts":[[2014,12]]},"published":{"date-parts":[[2014,12,1]]}}}