{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:12Z","timestamp":1789996512518,"version":"4.0.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEMS Microbiol Lett"],"published-print":{"date-parts":[[2014,5]]},"DOI":"10.1111\/1574-6968.12430","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T03:11:09Z","timestamp":1395717069000},"page":"27-36","source":"Crossref","is-referenced-by-count":50,"title":["Cold shock protein A plays an important role in the stress adaptation and virulence of<i>Brucella melitensis<\/i>"],"prefix":"10.1093","volume":"354","author":[{"given":"Zhen","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture; College of Veterinary Medicine; China Agricultural University; Beijing China"},{"name":"Animal Science and Technology College; Beijing University of Agriculture; Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangshan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological Sciences; Anyang Institute of Technology; Anyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture; College of Veterinary Medicine; China Agricultural University; Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,4,11]]},"reference":[{"key":"10.1111\/1574-6968.12430-BIB0001|fml12430-cit-0001","doi-asserted-by":"crossref","first-page":"7081","DOI":"10.1128\/jb.179.22.7081-7088.1997","article-title":"CspA, the major cold shock protein of Escherichia coli, negatively regulates its own gene expression","volume":"179","author":"Bae","year":"1997","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0002|fml12430-cit-0002","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1046\/j.1365-2958.1999.01284.x","article-title":"Characterization of Escherichia coli cspE, whose product negatively regulates transcription of cspA, the gene for the major cold shock protein","volume":"31","author":"Bae","year":"1999","journal-title":"Mol Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0003|fml12430-cit-0003","first-page":"363","article-title":"Macrophages and Brucella","volume":"60","author":"Baldwin","year":"1994","journal-title":"Immunol Ser"},{"key":"10.1111\/1574-6968.12430-BIB0004|fml12430-cit-0004","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1128\/JB.05623-11","article-title":"The RNA chaperone Hfq independently coordinates expression of the VirB type IV secretion system and the LuxR-type regulator BabR in Brucella abortus 2308","volume":"194","author":"Caswell","year":"2012","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0005|fml12430-cit-0005","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mib.2003.11.001","article-title":"Organelle robbery: Brucella interactions with the endoplasmic reticulum","volume":"7","author":"Celli","year":"2004","journal-title":"Curr Opin Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0006|fml12430-cit-0006","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.2460\/ajvr.1991.52.10.1658","article-title":"Effect of endocytic and metabolic inhibitors on the internalization and intracellular growth of Brucella abortus in Vero cells","volume":"52","author":"Detilleux","year":"1991","journal-title":"Am J Vet Res"},{"key":"10.1111\/1574-6968.12430-BIB0007|fml12430-cit-0007","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1007\/PL00012513","article-title":"Bacterial cold-shock proteins","volume":"59","author":"Ermolenko","year":"2002","journal-title":"Cell Mol Life Sci"},{"key":"10.1111\/1574-6968.12430-BIB0008|fml12430-cit-0008","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1462-5822.2005.00502.x","article-title":"The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection","volume":"7","author":"Fretin","year":"2005","journal-title":"Cell Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0009|fml12430-cit-0009","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1073\/pnas.87.1.283","article-title":"Major cold shock protein of Escherichia coli","volume":"87","author":"Goldstein","year":"1990","journal-title":"P Natl Acad Sci USA"},{"key":"10.1111\/1574-6968.12430-BIB0010|fml12430-cit-0010","doi-asserted-by":"crossref","first-page":"4611","DOI":"10.1128\/jb.178.15.4611-4619.1996","article-title":"Cold shock stress-induced proteins in Bacillus subtilis","volume":"178","author":"Graumann","year":"1996","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0011|fml12430-cit-0011","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1046\/j.1365-2958.1997.5121878.x","article-title":"A family of cold shock proteins in Bacillus subtilis is essential for cellular growth and for efficient protein synthesis at optimal and low temperatures","volume":"25","author":"Graumann","year":"1997","journal-title":"Mol Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0012|fml12430-cit-0012","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1074\/jbc.272.1.196","article-title":"CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone","volume":"272","author":"Jiang","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1111\/1574-6968.12430-BIB0013|fml12430-cit-0013","doi-asserted-by":"crossref","first-page":"4048","DOI":"10.1128\/IAI.01787-05","article-title":"Evaluation of protection afforded by Brucella abortus and Brucella melitensis unmarked deletion mutants exhibiting different rates of clearance in BALB\/c mice","volume":"74","author":"Kahl-McDonagh","year":"2006","journal-title":"Infect Immun"},{"key":"10.1111\/1574-6968.12430-BIB0014|fml12430-cit-0014","first-page":"800","article-title":"pBBR1MCS: a broad-host-range cloning vector","volume":"16","author":"Kovach","year":"1994","journal-title":"Biotechniques"},{"key":"10.1111\/1574-6968.12430-BIB0015|fml12430-cit-0015","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0923-2508(97)87645-0","article-title":"Response of Brucella suis 1330 and B.\u00a0canis RM6\/66 to growth at acid pH and induction of an adaptive acid tolerance response","volume":"148","author":"Kulakov","year":"1997","journal-title":"Res Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0016|fml12430-cit-0016","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0882-4010(92)90113-3","article-title":"Brucella abortus deficient in copper\/zinc superoxide dismutase is virulent in BALB\/c mice","volume":"12","author":"Latimer","year":"1992","journal-title":"Microb Pathog"},{"key":"10.1111\/1574-6968.12430-BIB0017|fml12430-cit-0017","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1128\/IAI.63.4.1409-1414.1995","article-title":"Protein synthesis in Brucella abortus induced during macrophage infection","volume":"63","author":"Lin","year":"1995","journal-title":"Infect Immun"},{"key":"10.1111\/1574-6968.12430-BIB0018|fml12430-cit-0018","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.vetmic.2012.03.022","article-title":"OtpR regulated the growth, cell morphology of B.\u00a0melitensis and tolerance to \u03b2-lactam agents","volume":"159","author":"Liu","year":"2012","journal-title":"Vet Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0019|fml12430-cit-0019","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCt method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1111\/1574-6968.12430-BIB0020|fml12430-cit-0020","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1600-065X.2010.00982.x","article-title":"An evolutionary strategy for a stealthy intracellular Brucella pathogen","volume":"240","author":"Martirosyan","year":"2011","journal-title":"Immunol Rev"},{"key":"10.1111\/1574-6968.12430-BIB0021|fml12430-cit-0021","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0378-1119(00)00071-8","article-title":"PCR-mediated gene replacement in Escherichia coli","volume":"246","author":"Murphy","year":"2000","journal-title":"Gene"},{"key":"10.1111\/1574-6968.12430-BIB0022|fml12430-cit-0022","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1128\/jb.178.10.2994-2997.1996","article-title":"A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli","volume":"178","author":"Nakashima","year":"1996","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0023|fml12430-cit-0023","first-page":"125","article-title":"Recent developments in bacterial cold-shock response","volume":"6","author":"Phadtare","year":"2004","journal-title":"Curr Issues Mol Biol"},{"key":"10.1111\/1574-6968.12430-BIB0024|fml12430-cit-0024","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1128\/JB.183.4.1205-1214.2001","article-title":"Role of CspC and CspE in regulation of expression of RpoS and UspA, the stress response proteins in Escherichia coli","volume":"183","author":"Phadtare","year":"2001","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0025|fml12430-cit-0025","doi-asserted-by":"crossref","first-page":"7007","DOI":"10.1128\/JB.186.20.7007-7014.2004","article-title":"Genome-wide transcriptional analysis of the cold shock response in wild-type and cold-sensitive, quadruple-csp-deletion strains of Escherichia coli","volume":"186","author":"Phadtare","year":"2004","journal-title":"J Bacteriol"},{"key":"10.1111\/1574-6968.12430-BIB0026|fml12430-cit-0026","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.1128\/IAI.67.8.4041-4047.1999","article-title":"Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages","volume":"67","author":"Porte","year":"1999","journal-title":"Infect Immun"},{"key":"10.1111\/1574-6968.12430-BIB0027|fml12430-cit-0027","doi-asserted-by":"crossref","first-page":"5274","DOI":"10.1128\/IAI.64.12.5274-5283.1996","article-title":"Identification and comparison of macrophage induced proteins and proteins induced under various stress conditions in Brucella abortus","volume":"64","author":"Rafie-Kolpin","year":"1996","journal-title":"Infect Immun"},{"key":"10.1111\/1574-6968.12430-BIB0028|fml12430-cit-0028","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1046\/j.1365-2958.1999.01629.x","article-title":"The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice","volume":"34","author":"Robertson","year":"1999","journal-title":"Mol Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0029|fml12430-cit-0029","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1046\/j.1365-2958.2000.01726.x","article-title":"The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB\/c mice","volume":"35","author":"Robertson","year":"2000","journal-title":"Mol Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0030|fml12430-cit-0030","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1128\/IAI.01125-12","article-title":"Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus","volume":"81","author":"Roset","year":"2013","journal-title":"Infect Immun"},{"key":"10.1111\/1574-6968.12430-BIB0031|fml12430-cit-0031","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1128\/AEM.02154-08","article-title":"Role of cold shock proteins in growth of Listeria monocytogenes under cold and osmotic stress conditions","volume":"75","author":"Schmid","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0032|fml12430-cit-0032","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2007.11.023","article-title":"Brucella: a pathogen without classic virulence genes","volume":"129","author":"Seleem","year":"2008","journal-title":"Vet Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0033|fml12430-cit-0033","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/CVI.00573-12","article-title":"In vivo difference in the virulence, pathogenicity, and induced protective immunity of wboA mutants from genetically different parent Brucella spp","volume":"20","author":"Wang","year":"2013","journal-title":"Clin Vaccine Immunol"},{"key":"10.1111\/1574-6968.12430-BIB0034|fml12430-cit-0034","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1098\/rstb.2002.1078","article-title":"Coping with the cold: the cold shock response in the Gram-positive soil bacterium Bacillus subtilis","volume":"357","author":"Weber","year":"2002","journal-title":"Philos Trans R Soc Lond"},{"key":"10.1111\/1574-6968.12430-BIB0035|fml12430-cit-0035","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/1471-2180-6-102","article-title":"Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival","volume":"6","author":"Wu","year":"2006","journal-title":"BMC Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0036|fml12430-cit-0036","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1046\/j.1365-2958.1998.00683.x","article-title":"The CspA family in Escherichia coli: multiple gene duplication for stress adaptation","volume":"27","author":"Yamanaka","year":"1998","journal-title":"Mol Microbiol"},{"key":"10.1111\/1574-6968.12430-BIB0037|fml12430-cit-0037","doi-asserted-by":"crossref","first-page":"570","DOI":"10.7150\/ijbs.5.570","article-title":"Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance","volume":"5","author":"Zhang","year":"2009","journal-title":"Int J Biol Sci"}],"container-title":["FEMS Microbiology Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsle\/article-pdf\/354\/1\/27\/19125326\/354-1-27.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T09:32:39Z","timestamp":1716629559000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsle\/article-lookup\/doi\/10.1111\/1574-6968.12430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,11]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,5]]}},"URL":"https:\/\/doi.org\/10.1111\/1574-6968.12430","relation":{},"ISSN":["0378-1097"],"issn-type":[{"value":"0378-1097","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4,11]]}}}