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In this study, detoxification activity of the epsilon GST SlGSTE1 in <jats:italic>Spodoptera litura<\/jats:italic> was analyzed for several insecticides and heavy metals. SlGSTE1 was significantly up\u2010regulated by chlorpyrifos and xanthotoxin in the midgut of <jats:italic>S. litura<\/jats:italic>. The recombinant SlGSTE1 had <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> (reaction rate of the enzyme saturated with the substrate) and <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> (michaelis constant and equals to the substrate concentration at half of the maximum reaction rate of the enzyme) values of 27.95\u00a0\u00b1\u00a00.88 <jats:italic>\u03bc<\/jats:italic>mol\/min\/mg and 0.87\u00a0\u00b1\u00a00.028\u00a0mmol\/L for glutathione, respectively, and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> and <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values of 22.96\u00a0\u00b1\u00a00.78 <jats:italic>\u03bc<\/jats:italic>mol\/min\/mg and 0.83\u00a0\u00b1\u00a00.106 mmol\/L for 1\u2010chloro\u20102,4\u2010dinitrobenzene, respectively. <jats:italic>In vitro<\/jats:italic> enzyme indirect activity assay showed that the recombinant SlGSTE1 possessed high binding activities to the insecticides chlorpyrifos, deltamethrin, malathion, phoxim and dichloro\u2010diphenyl\u2010trichloroethane (DDT). SlGSTE1 showed higher binding activity to toxic heavy metals cadmium, chromium and lead than copper and zinc that are required for insect normal growth. Western blot analysis showed that SlGSTE1 was induced in the gut of larvae fed with chlorpyrifos or cadmium. SlGSTE1 also showed high peroxidase activity. All the results together indicate that SlGSTE1 may play an important role in the gut of <jats:italic>S. litura<\/jats:italic> to protect the insect from the toxic effects of these compounds and heavy metals.<\/jats:p>","DOI":"10.1111\/1744-7917.12142","type":"journal-article","created":{"date-parts":[[2014,5,26]],"date-time":"2014-05-26T15:48:38Z","timestamp":1401119318000},"page":"503-511","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Detoxification of insecticides, allechemicals and heavy metals by glutathione <i>S<\/i>\u2010transferase SlGSTE1 in the gut of <i>Spodoptera litura<\/i>"],"prefix":"10.1111","volume":"22","author":[{"given":"Zhi\u2010Bin","family":"Xu","sequence":"first","affiliation":[{"name":"Laboratory of Molecular and Developmental Entomology Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life Sciences South China Normal University  Guangzhou 510631 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