{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:22:36Z","timestamp":1773663756089,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T00:00:00Z","timestamp":1415836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"crossref","award":["BRG5580013"],"award-info":[{"award-number":["BRG5580013"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study characterizes the colonization and composition of bacterial flora in dwarf Asian honeybee (<jats:italic>Apis florea<\/jats:italic>) larvae and compares bacterial diversity and distribution among different sampling locations. <jats:italic>A. florea<\/jats:italic> larvae were collected from 3 locations in Chiang Mai province, Thailand. Bacterial DNA was extracted from each larva using the phenol\u2013chloroform method. Denaturing gradient gel electrophoresis was performed, and the dominant bands were excised from the gels, cloned, and sequenced for bacterial species identification. The result revealed similarities of bacterial community profiles in each individual colony, but differences between colonies from the same and different locations. <jats:italic>A. florea<\/jats:italic> larvae harbor bacteria belonging to 2 phyla (Firmicutes and Proteobacteria), 5 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacilli, and Clostridia), 6 genera (Clostridium, Gilliamella, Melissococcus, Lactobacillus, Saccharibacter, and Snodgrassella), and an unknown genus from uncultured bacterial species. The classes with the highest abundance of bacteria were Alphaproteobacteria (34%), Bacilli (25%), Betaproteobacteria (11%), Gammaproteobacteria (10%), and Clostridia (8%), respectively. Similarly, uncultured bacterial species were identified (12%). Environmental bacterial species, such as <jats:italic>Saccharibacter floricola<\/jats:italic>, were also found. This is the first study in which sequences closely related to <jats:italic>Melissococcus plutonius<\/jats:italic>, the causal pathogen responsible for European foulbrood, have been identified in Thai <jats:italic>A. florea<\/jats:italic> larvae.<\/jats:p>","DOI":"10.1111\/1744-7917.12155","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T08:39:08Z","timestamp":1415867948000},"page":"606-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Bacterial community structure in <i>Apis florea<\/i> larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing"],"prefix":"10.1111","volume":"22","author":[{"given":"Prakaimuk","family":"Saraithong","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Chiang Mai University  Chiang Mai 50200 Thailand"},{"name":"Department of Basic Science and Craniofacial Biology New York University College of Dentistry  New York 10010 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Basic Science and Craniofacial Biology New York University College of Dentistry  New York 10010 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanokporn","family":"Saenphet","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Chiang Mai University  Chiang Mai 50200 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Basic Science and Craniofacial Biology New York University College of Dentistry  New York 10010 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panuwan","family":"Chantawannakul","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Chiang Mai University  Chiang Mai 50200 Thailand"},{"name":"Materials Science Research Center, Faculty of Science Chiang Mai University  Chiang Mai 50200 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-012-2188-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(90)90090-S"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00040-011-0194-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(74)90192-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1983.tb02648.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.12.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1146498"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01336-10"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.49.061802.123416"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2011.01216.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202970109"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12025"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2009.06.016"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-004-0188-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30744-3_10"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7216-7223.2003"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057169"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5344\/ajev.1996.47.1.1","article-title":"Relationship between sluggish fermentations and the antagonism of yeast by lactic acid bacteria","volume":"47","author":"Huang Y.C.","year":"1996","journal-title":"American Journal of Enology and Viticulture"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:19980403"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2003.08.007"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02911-0"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12209"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110474108"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.044875-0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9320-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2010.04959.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07810-11"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/0005772X.1993.11099183"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12302"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.025940-0"},{"key":"e_1_2_7_32_1","first-page":"573","article-title":"Field study results on the probability and risk of horizontal gene transfer from transgenic herbicide\u2010resistant oil seed rape pollen to gut bacteria of bees","volume":"75","author":"Mohr K.I.","year":"2007","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036393"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj2000.6441382x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-012-0186-4"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-008-9202-0"},{"key":"e_1_2_7_37_1","first-page":"167","article-title":"Revised distribution of three recently recognized species of honey bees in Asia","volume":"15","author":"Otis G.W.","year":"1991","journal-title":"Honeybee Science"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.1996.11100909"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-009-9595-8"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100304108"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4159\/9780674043404-003","volume-title":"The Wisdom of the Hive: The Social Physiology of Honey Bee Colonies","author":"Seeley T.D.","year":"1995"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.11.029"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00029-9"},{"key":"e_1_2_7_44_1","first-page":"81","article-title":"A contribution to the biology and behavior of the dwarf bee, A. florea F. and its economic importance in Kutch, Gujarat, India","volume":"58","author":"Soman A.G.","year":"1996","journal-title":"Indian Bee Journal"},{"key":"e_1_2_7_45_1","first-page":"907","article-title":"Influence of the diet components on the symbiotic microorganisms community in hindgut of","volume":"71","author":"Tanaka H.","year":"2006","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_7_46_1","first-page":"1710","article-title":"Characterization of gut associated bacteriain larva and adults of the Southern pine beetle, Dendroctonus frontalis Zimmermann","volume":"35","author":"Vasanthakuma A.","year":"2006","journal-title":"Molecular Ecology and Evolution"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033188"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072106"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2009.07.005"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2008.03867.x"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1744-7917.12155","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.12155","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:14:41Z","timestamp":1695975281000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.12155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,13]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/1744-7917.12155"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.12155","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,13]]},"assertion":[{"value":"2014-06-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-11-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}