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To achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, <jats:italic>Arabidopsis<\/jats:italic>, and rice plants expressing the fusion protein, AaIT\/GNA, in which an insecticidal scorpion venom neurotoxin (<jats:italic>Androctonus australis<\/jats:italic> toxin, AaIT) is fused to snowdrop lectin (<jats:italic>Galanthus nivalis<\/jats:italic> agglutinin, GNA). Compared with transgenic tobacco and <jats:italic>Arabidopsis<\/jats:italic> plants expressing AaIT or GNA, transgenic plants expressing AaIT\/GNA exhibited increased resistance and toxicity to one chewing pest, the cotton bollworm, <jats:italic>Helicoverpa armigera<\/jats:italic>. Transgenic tobacco and rice plants expressing AaIT\/GNA showed increased resistance and toxicity to two sucking pests, the whitefly, <jats:italic>Bemisia tabaci<\/jats:italic>, and the rice brown planthopper, <jats:italic>Nilaparvata lugens<\/jats:italic>, respectively. Moreover, in the field, transgenic rice plants expressing AaIT\/GNA exhibited a significant improvement in grain yield when infested with <jats:italic>N. lugens<\/jats:italic>. This study shows that expressing the AaIT\/GNA fusion protein in transgenic plants can be a useful approach for controlling pests, particularly sucking pests which are not susceptible to the toxin in Bt crops.<\/jats:p>","DOI":"10.1111\/1744-7917.12203","type":"journal-article","created":{"date-parts":[[2015,1,16]],"date-time":"2015-01-16T07:53:25Z","timestamp":1421394805000},"page":"265-276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Transgenic plants expressing the AaIT\/GNA fusion protein show increased resistance and toxicity to both chewing and sucking pests"],"prefix":"10.1111","volume":"23","author":[{"given":"Shu\u2010Min","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai 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