{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:00:25Z","timestamp":1761156025170},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270691, 31170609"],"award-info":[{"award-number":["31270691, 31170609"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A recombinant <jats:italic>Helicoverpa armigera<\/jats:italic> nucleopolyhedrovirus (HearNPV) expressing the insect\u2010selective neurotoxin (<jats:italic>RjAa17f<\/jats:italic>) from Cuban scorpion <jats:italic>Rhopalurus junceus<\/jats:italic> was constructed by replacing the UDP\u2010glucosyltransferase gene (<jats:italic>egt<\/jats:italic>) using \u03bb\u2010red homologous recombination system. Another <jats:italic>egt<\/jats:italic> deleted control HearNPV was constructed in a similar way by inserting <jats:italic>egfp<\/jats:italic> gene into the <jats:italic>egt<\/jats:italic> locus. One\u2010step viral growth curve and viral DNA replication curve analysis confirmed that the recombination did not affect the viral growth and DNA replication in host cells. There is no discernable difference in occlusion\u2010body morphogenesis between RjAa17f\u2010HearNPV, Egfp\u2010HearNPV and HZ8\u2010HearNPV, which was confirmed by transmission electron microscopy analysis. However, the insecticidal activity of RjAa17f\u2010HearNPV is enhanced against the third instar <jats:italic>H. armigera<\/jats:italic> larvae according to the bioassay on virulence comparison. There is a dramatic reduction (56.9%) in median lethal dose (LD<jats:sub>50<\/jats:sub>) and also a reduction (13.4%) in median survival time (ST<jats:sub>50<\/jats:sub>) for the recombinant RjAa17f\u2010HearNPV compared to the HZ8\u2010HearNPV, but only a 27.5% reduction in LD<jats:sub>50<\/jats:sub> and 10.1% reduction in ST<jats:sub>50<\/jats:sub> value when Egfp\u2010HearNPV is compared with HZ8\u2010HearNPV. The daily diet consumption analysis showed that the RjAa17f\u2010HearNPV was able to inhibit the infected larvae feeding compared with the <jats:italic>egt<\/jats:italic> minus HearNPV. These results demonstrated that this novel recombinant RjAa17f\u2010HearNPV could improve the insecticidal effect against its host insects and <jats:italic>RjAa17f<\/jats:italic> could be a considerable candidate for other recombinant baculovirus constructions.<\/jats:p>","DOI":"10.1111\/1744-7917.12289","type":"journal-article","created":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T09:46:46Z","timestamp":1446803206000},"page":"397-408","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Recombinant <i>Helicoverpa armigera<\/i> nucleopolyhedrovirus with arthropod\u2010specific neurotoxin gene <i>RjAa17f<\/i> from <i>Rhopalurus junceus<\/i> enhances the virulence against the host larvae"],"prefix":"10.1111","volume":"24","author":[{"given":"Huan","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas  Yangling Shaanxi China"},{"name":"Key Laboratory 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