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Some of the previous studies involved heat response studies in terms of survival and heat shock protein expression, which are regulated to protect other proteins against environmental stress conditions. In the present study, we characterize the impedance profile with the xCELLigence Real\u2010Time Cell Analyzer and study the effect of increased temperature in cell growth and viability in the cell line BCIRL\u2010TcA\u2010CLG1 (TcA) of <jats:italic>T. castaneum<\/jats:italic>. This novel system measures cells behavior in real time and is applied for the first time to insect cells. Additionally, cells are exposed to heat shock, increased salinity, acidic pH and UV\u2010A light with the aim of measuring the expression levels of <jats:italic>Hsp27<\/jats:italic>, <jats:italic>Hsp68a<\/jats:italic>, and <jats:italic>Hsp83<\/jats:italic> genes. Results show a high thermotolerance of TcA in terms of cell growth and viability. This result is likely related to gene expression results in which a significant up\u2010regulation of all studied Hsp genes is observed after 1 h of exposure to 40 \u00b0C and UV light. All 3 genes show similar expression patterns, but <jats:italic>Hsp27<\/jats:italic> seems to be the most affected. The results of this study validate the RTCA method and reveal the utility of insect cell lines, real\u2010time analysis and gene expression studies to better understand the physiological response of insect cells, with potential applications in different fields of biology such as conservation biology and pest management.<\/jats:p>","DOI":"10.1111\/1744-7917.12306","type":"journal-article","created":{"date-parts":[[2015,12,17]],"date-time":"2015-12-17T15:41:49Z","timestamp":1450366909000},"page":"358-370","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Real\u2010time cell analysis and heat shock protein gene expression in the TcA <i>Tribolium castaneum<\/i> cell line in response to environmental stress conditions"],"prefix":"10.1111","volume":"24","author":[{"given":"Andr\u00e9s","family":"Garc\u00eda\u2010Reina","sequence":"first","affiliation":[{"name":"Department of Zoology and Physical Anthropology University 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