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In the present study, we identified and characterized the <jats:italic>Masculinizer<\/jats:italic> (<jats:italic>Masc<\/jats:italic>) gene in a Noctuidae pest species, <jats:italic>Agrotis ipsilon<\/jats:italic>. Sequence analysis revealed that <jats:italic>AiMasc<\/jats:italic> encodes a protein of 658 amino acids that has two CCCH\u2010type zinc finger domains and two conserved cysteine residues (Cys\u2010277 and Cys\u2010280). We assessed the masculinizing activity of <jats:italic>AiMasc<\/jats:italic> in BmN cells and found that <jats:italic>AiMasc<\/jats:italic> induced expression of the male\u2010specific <jats:italic>doublesex<\/jats:italic> isoform. Disruption of <jats:italic>Masc<\/jats:italic> via clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR\u2010associated protein 9 (Cas9) in <jats:italic>A. ipsilon<\/jats:italic> caused abnormalities in abdominal segments and external genitalia, resulting in male\u2010specific sterility. These results suggest that <jats:italic>Masc<\/jats:italic> participates in the process of sex determination in <jats:italic>A. ipsilon<\/jats:italic>. Successful identification of sex\u2010determination gene in a pest species may enable the development of novel genetic approaches for pest control.<\/jats:p>","DOI":"10.1111\/1744-7917.12635","type":"journal-article","created":{"date-parts":[[2018,8,8]],"date-time":"2018-08-08T14:33:11Z","timestamp":1533738791000},"page":"1037-1044","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The <i>Masc<\/i> gene product controls masculinization in the black cutworm, <i>Agrotis ipsilon<\/i>"],"prefix":"10.1111","volume":"26","author":[{"given":"Yao\u2010Hui","family":"Wang","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology  Shanghai China"},{"name":"Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science &amp; Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010En","family":"Chen","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Science East China Normal University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Xu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang\u2010Qi","family":"Fang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Ying","family":"Niu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science &amp; 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