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In the broader context of insect development and pest control strategies, this study investigates how a new ADH5 from <jats:italic>Helicoverpa armigera<\/jats:italic> (<jats:italic>Ha<\/jats:italic>ADH5) regulates the expression of <jats:italic>CYP6B6<\/jats:italic>, a gene involved in molting and metamorphosis, in response to 2\u2010TD treatment. Cloning of the <jats:italic>HaADH5<\/jats:italic> complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 kD. <jats:italic>Ha<\/jats:italic>ADH5 protein was purified in the <jats:italic>Escherichia coli<\/jats:italic> Transetta (pET32a\u2010<jats:italic>HaADH5<\/jats:italic>) strain using a prokaryotic expression system. The ability of <jats:italic>Ha<\/jats:italic>ADH5 protein to interact with the 2\u2010TD responsive region within the promoter of <jats:italic>CYP6B6<\/jats:italic> was confirmed by an <jats:italic>in vitro<\/jats:italic> electrophoretic mobility shift assay and transcription activity validation in yeast. Finally, the expression levels of both <jats:italic>HaADH5<\/jats:italic> and <jats:italic>CYP6B6<\/jats:italic> were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg\/g 2\u2010TD artificial diet. These results indicate that upon 2\u2010TD treatment of cotton bollworm, <jats:italic>Ha<\/jats:italic>ADH5 regulates the expression of <jats:italic>CYP6B6<\/jats:italic> by interacting with its promoter. As <jats:italic>Ha<\/jats:italic>ADH5 regulation of <jats:italic>CYP6B6<\/jats:italic> expression may contribute to the larval xenobiotic detoxification, molting and metamorphosis, <jats:italic>Ha<\/jats:italic>ADH5 is a candidate target for controlling the growth and development of cotton bollworm.<\/jats:p>","DOI":"10.1111\/1744-7917.12720","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T13:26:15Z","timestamp":1566912375000},"page":"1053-1066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Alcohol dehydrogenase 5 of <i>Helicoverpa armigera<\/i> interacts with the <i>CYP6B6<\/i> promoter in response to 2\u2010tridecanone"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5687-9286","authenticated-orcid":false,"given":"Jie","family":"Zhao","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic 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