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Depending on the products formed, aaNATs are involved in a variety of physiological functions. In the yellow fever mosquito, <jats:italic>Aedes aegypti<\/jats:italic>, a number of aaNATs and aaNAT\u2010like proteins have been reported. However, the primary function of each individual aaNAT is yet to be identified. In this study we investigated the function of <jats:italic>Ae. aegypti<\/jats:italic> aaNAT1 (Ae\u2010aaNAT1) in cuticle pigmentation and development of morphology. Ae\u2010aaNAT1 transcripts were detected at all stages of development with highest expressions after pupation and right before adult eclosion. Ae\u2010aaNAT1 mutant mosquitoes generated using clustered regularly interspaced palindromic repeats (CRISPR) \u2013 CRISPR\u2010associated protein 9 had no obvious effect on larval and pupal development. However, the mutant mosquitoes exhibited a roughened exoskeletal surface, darker cuticles, and color pattern changes suggesting that Ae\u2010aaNAT1 plays a role in development of the morphology and pigmentation of <jats:italic>Ae. aegypti<\/jats:italic> adult cuticles. The mutant also showed less blood feeding efficiency and lower fecundity when compared with the wild\u2010type. The mutation of <jats:italic>Ae\u2010aaNAT1<\/jats:italic> influenced expression of genes involved in cuticle formation. In summary, <jats:italic>Ae\u2010aaNAT1<\/jats:italic> mainly functions on cuticular pigmentation and also affects blood feeding efficiency and fecundity.<\/jats:p>","DOI":"10.1111\/1744-7917.12895","type":"journal-article","created":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T13:49:43Z","timestamp":1608990583000},"page":"1591-1600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Arylalkalamine N\u2010acetyltransferase\u20101 functions on cuticle pigmentation in the yellow fever mosquito, <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"28","author":[{"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Pharmaceutical Sciences Hainan University  Haikou 570228 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