{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:03:56Z","timestamp":1783577036148,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2016RA4008"],"award-info":[{"award-number":["2016RA4008"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011798","name":"Ministry of Agriculture and Rural Affairs of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011798","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31620103915"],"award-info":[{"award-number":["31620103915"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31830074"],"award-info":[{"award-number":["31830074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To achieve successful development, female parasitoids, while laying eggs, introduce various virulence factors, mainly venoms, into host insects to manipulate their physiology. Although numerous studies have been conducted to characterize the components of venoms that regulate host immune responses, few systematic investigations have been conducted on the roles of venom proteins in host metabolic regulation. In this investigation, we characterized a novel venom protein in <jats:italic>Pachycrepoideus vindemiae<\/jats:italic> called glucose\u20106\u2010phosphate dehydrogenase (PvG6PDH) and showed it has a vital role in regulating host carbohydrate metabolism. PvG6PDH encodes 510 amino acids and features a signal peptide and two conserved \u201cG6PDH\u201d domains. Multiple sequence alignment showed it has high amino acid identity with G6PDH from other pteromalids, and quantitative polymerase chain reaction analysis and immunofluorescent staining demonstrated a significantly higher expression of PvG6PDH in the venom apparatus compared with the carcass. We report that PvG6PDH contributes to parasitism by inhibiting the glucose\u20106\u2010phosphate (G6P) metabolism of host <jats:italic>Drosophila melanogaster<\/jats:italic>, as demonstrated by PvG6PDH injection and RNA interference analysis. Further tests revealed that the accumulation of host G6P was caused by the transcriptional inhibition of G6P\u2010metabolism\u2010related genes. These findings greatly contribute to our understanding of venom\u2010mediated host metabolic regulation, further laying the foundation for the development of venom proteins as biological agents for pest control.<\/jats:p>","DOI":"10.1111\/1744-7917.12935","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T16:52:55Z","timestamp":1635785575000},"page":"399-410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A venom protein of ectoparasitoid <i>Pachycrepoideus vindemiae<\/i>, PvG6PDH, contributes to parasitism by inhibiting host glucose\u20106\u2010phosphate metabolism"],"prefix":"10.1111","volume":"29","author":[{"given":"Lei","family":"Yang","sequence":"first","affiliation":[{"name":"Hainan University  Haikou 570228 China"},{"name":"State Key Laboratory of Rice Biology &amp; Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Qiu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology &amp; Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology &amp; Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoying","family":"Wu","sequence":"additional","affiliation":[{"name":"Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4937-8867","authenticated-orcid":false,"given":"Gongyin","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology &amp; Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0036-z"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120709-144849"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006431"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.49.061802.123324"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0218301"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku340"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2017.07.002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01546-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0013873816050018"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583157.2012.731497"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153622"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583157.2011.594951"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183525"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jipm\/pmz012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx922"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.54.110807.090556"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv362"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2009.09.001"},{"key":"e_1_2_8_21_1","first-page":"655","article-title":"Parasit\u00f3ides de est\u00e1gios imaturos de d\u00edpteros sinantr\u00f3picos coletados em v\u00e1rios ambientes em Itumbiara\u2010GO","volume":"22","author":"Marchiori C.H.","year":"2000","journal-title":"Acta Scientiarum"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1519-69842007000300025"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.03516"},{"key":"e_1_2_8_24_1","first-page":"228","article-title":"Hosts of the parasitoid Pachycrepoideus vindemmiae (Rondani) (Hymenoptera: Pteromalidae) of medical\u2010veterinary and economic importance collected in the state of Goi\u00e1s, Brazil","volume":"1","author":"Marchiori C.H.","year":"2013","journal-title":"The American Journal of Medicine"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782003000100029"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12967"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins7072385"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-014-0697-z"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.51.110104.151029"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21182"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21405"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2017.02.011"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2012.738274"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2015.02.003"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7917.2012.01519.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00824"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5755"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.336"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01282"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00009"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/520926"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.12935","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.12935","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.12935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T23:23:19Z","timestamp":1693005799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.12935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/1744-7917.12935"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.12935","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2021-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}