{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T02:15:07Z","timestamp":1769739307256,"version":"3.49.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Krotzkopf verkehrt (kkv) is a key enzyme that catalyzes the synthesis of chitin, an important component of the <jats:italic>Drosophila<\/jats:italic> epidermis, trachea, and other tissues. Here, we report the use of comprehensive RNA interference (RNAi) analyses to search for <jats:italic>kkv<\/jats:italic> transcriptional regulators. A cell\u2010based RNAi screen identified 537 candidate <jats:italic>kkv<\/jats:italic> regulators on a genome\u2010wide scale. Subsequent use of transgenic <jats:italic>Drosophila<\/jats:italic> lines expressing RNAi constructs enabled <jats:italic>in vivo<\/jats:italic> validation, and we identified six genes as potential <jats:italic>kkv<\/jats:italic> transcriptional regulators. Weakening of the <jats:italic>kkv<\/jats:italic>DsRed signal, an <jats:italic>in vivo<\/jats:italic> reporter indicating <jats:italic>kkv<\/jats:italic> promoter activity, was observed when the expression of Akirin, NFAT, 48 related 3 (<jats:italic>Fer3<\/jats:italic>), or Autophagy\u2010related 101(Atg101) was knocked down in <jats:italic>Drosophila<\/jats:italic> at the 3rd\u2010instar larval stage; whereas we observed disoriented taenidial folds on larval tracheae when Lines (<jats:italic>lin<\/jats:italic>) or Autophagy\u2010related 3 (<jats:italic>Atg3<\/jats:italic>) was knocked down in the tracheae. Fer3, in particular, has been shown to be an important factor in the activation of <jats:italic>kkv<\/jats:italic> transcription via specific binding with the <jats:italic>kkv<\/jats:italic> promoter. The genes involved in the chitin synthesis pathway were widely affected by the downregulation of <jats:italic>Fer3<\/jats:italic>. Furthermore, Atg101, Atg3, Akirin, Lin, NFAT, Pnr, and Abd\u2010A showed that the potential complex mechanism of <jats:italic>kkv<\/jats:italic> transcription is regulated by an interaction network with bithorax complex components. Our study revealed the hitherto unappreciated diversity of modulators impinging on <jats:italic>kkv<\/jats:italic> transcription and opens new avenues in the study of <jats:italic>kkv<\/jats:italic> regulation and chitin biosynthesis.<\/jats:p>","DOI":"10.1111\/1744-7917.12954","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T12:05:29Z","timestamp":1628165129000},"page":"614-630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genome\u2010wide <i>in vitro<\/i> and <i>in vivo<\/i> RNAi screens reveal Fer3 to be an important regulator of <i>kkv<\/i> transcription in <i>Drosophila<\/i>"],"prefix":"10.1111","volume":"29","author":[{"given":"Xiangzhao","family":"Yue","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"},{"name":"College of Life Sciences Shangrao Normal University  Shangrao 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