{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:10:51Z","timestamp":1791868251536,"version":"4.3.4"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T00:00:00Z","timestamp":1634256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The two\u2010spotted spider mite <jats:italic>Tetranychus urticate<\/jats:italic> is an important agricultural pest worldwide. It is extremely polyphagous and has developed resistance to many pesticides. Here, we assessed the pesticide resistance of seven field populations of <jats:italic>T. urticae<\/jats:italic> in China, their target site mutations and the activities of their detoxification enzymes. The results showed that abamectin and the traditional pesticides pyridaben, profenofos and bifenthrin had higher resistance or lower toxicity than more recently developed pesticides including chlorfenapyr, spinetoram, cyflumetofen, cyenopyrafen, bifenazate and B\u2010azolemiteacrylic. The frequency of point mutations related to abamectin resistance, G314D in the glutamate\u2010gated chloride channel 1 (GluCl1) and G326E in GluCl3, ranged 47%\u201370% and 0%\u201397%, respectively. The frequency of point mutations in A1215D and F1538I of the voltage\u2010gated sodium channel gene (VGSC), which may increase resistance to pyrethroids, ranged 88%\u2013100% and 10%\u2013100%, respectively. For target sites related to organophosphate resistance, mutation frequencies ranged 25%\u201392% for G119S and 0%\u201323% for A201S in the acetycholinesterase gene (Ace). Mutation G126S in the bifenazate resistance\u2010related cytochrome b gene (Cytb) was observed in three of the seven <jats:italic>T. urticae<\/jats:italic> populations. Higher activities of detoxification enzymes (P450, GST, CarEs and UGTs) were observed in two <jats:italic>T. urticae<\/jats:italic> populations, with significant difference in the XY\u2010SX population. These results provide useful information on the status of pesticide resistance of <jats:italic>T. urticae<\/jats:italic> in China and suggest that <jats:italic>T. urticae<\/jats:italic> field populations may have multiple resistance mechanisms.<\/jats:p>","DOI":"10.1111\/1744-7917.12957","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T09:30:45Z","timestamp":1627291845000},"page":"827-839","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Frequencies and mechanisms of pesticide resistance in <i>Tetranychus urticae<\/i> field populations in China"],"prefix":"10.1111","volume":"29","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3508-6695","authenticated-orcid":false,"given":"Youjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3220-1072","authenticated-orcid":false,"given":"Wen","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4682-7241","authenticated-orcid":false,"given":"Zhaojiang","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5170-6781","authenticated-orcid":false,"given":"Shaoli","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing 100081 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T. Vanholme B. Van Pottelberge S. vanNieuwenhuyse P. Nauen R.andTirry L.et al. (2008) Mitochondrial heteroplasmy and the evolution of insecticide resistance: Non\u2010Mendelian inheritance in action. Proceedings of the National Academy of Sciences USA 105 5980\u20135985.","DOI":"10.1073\/pnas.0802224105"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.05.008"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.12.009"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1705"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485308006081"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5312"},{"key":"e_1_2_7_47_1","first-page":"102","article-title":"Sublethal effects of spinetoram on the two\u2010spotted spider mite, Tetranychus urticae (Acari: Tetranychidae)","volume":"132","author":"Wang L.","year":"2016","journal-title":"Systematic and Applied Acarology"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12637"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5109"},{"key":"e_1_2_7_50_1","first-page":"782","article-title":"CAPS marker monitoring abamectin resistance in Tetranychus urticae Koch","volume":"45","author":"Xu D.D.","year":"2018","journal-title":"Journal of Plant Protection"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2018.07.008"},{"key":"e_1_2_7_52_1","first-page":"33 pp","article-title":"Monitoring of pesticide resistance and the mutation frequencies of target genes in Tetranychus urticae","author":"Xu D.D.","year":"2019","journal-title":"Chinese Academy of Agricultural Sciences Publishing, Beijing, China"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6130"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.128007"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6215"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5831"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.12957","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.12957","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.12957","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T20:41:39Z","timestamp":1692823299000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.12957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,15]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/1744-7917.12957"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.12957","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,15]]},"assertion":[{"value":"2021-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}