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Here we describe the first<jats:italic>Wolbachia<\/jats:italic>infection from this group,<jats:italic>w<\/jats:italic>Clay isolated from<jats:italic>Scaptodrosophila claytoni<\/jats:italic>(van Klinken), a species from the east coast of Australia. The infection is polymorphic in natural populations, occurring at a frequency of around 6%\u201310%.<jats:italic>w<\/jats:italic>Clay causes male killing, producing female\u2010biased lines; most lines showed 100% male killing, though in 1 line it was &lt;80%. The lines need to be maintained through the introduction of males unless the infection is removed by tetracycline treatment.<jats:italic>w<\/jats:italic>Clay is transmitted at a high fidelity (98.6%) through the maternal lineage and has been stable in 2 laboratory lines across 24 generations, suggesting it is likely to persist in populations. The infection has not been previously described but is closely related to the male\u2010killing<jats:italic>Wolbachia<\/jats:italic>recently described from<jats:italic>Drosophila pandora<\/jats:italic>based on multilocus sequence typing and the<jats:italic>wsp<\/jats:italic>gene. Male\u2010killing<jats:italic>Wolbachia<\/jats:italic>are likely to be common in drosophilids but remain difficult to detect because the infections can often be at a low frequency.<\/jats:p>","DOI":"10.1111\/1744-7917.13000","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T13:14:45Z","timestamp":1641993285000},"page":"1401-1413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the first<i>Wolbachia<\/i>from the genus<i>Scaptodrosophila<\/i>, a male\u2010killer from the rainforest species<i>S. claytoni<\/i>"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7186-1260","authenticated-orcid":false,"given":"Kelly M.","family":"Richardson","sequence":"first","affiliation":[{"name":"School of Biosciences Bio21 Institute, The University of Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Schiffer","sequence":"additional","affiliation":[{"name":"Daintree Rainforest Observatory James Cook University Cape Tribulation Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perran A.","family":"Ross","sequence":"additional","affiliation":[{"name":"School of Biosciences Bio21 Institute, The University of Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua A.","family":"Thia","sequence":"additional","affiliation":[{"name":"School of Biosciences Bio21 Institute, The University of Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ary A.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"School of Biosciences Bio21 Institute, The University of Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00240.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/4102"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/4495"},{"key":"e_1_2_7_5_1","unstructured":"B\u00e4chli G.(2016) The database on Taxonomy of Drosophilidae.http:\/\/taxodros.uzh.ch\/."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00731-06"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3113.1978.tb00106.x"},{"key":"e_1_2_7_8_1","first-page":"147","article-title":"Culture methods for species of the Drosophila (Scaptodrosophila) coracina group","volume":"55","author":"Bock I.","year":"1980","journal-title":"Drosophila information service"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv494"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/367453a0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0049124104268644"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects10050126"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0471-x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.027854"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600852011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2001.1593"},{"key":"e_1_2_7_17_1","unstructured":"Geospiza Inc.(2004)FinchTV 1.4.0. 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