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Here, we analyzed how sublethal exposure of the promising vector\u2010control bioinsecticide spinetoram to <jats:italic>Aedes aegypti<\/jats:italic> larvae alter adult performance and susceptibility to dengue virus (DENV) infection. We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood\u2010feeding capacity than control females, apart from the extended immature development period. In terms of reproductive potential, although the F<jats:sub>0<\/jats:sub> generation produced a similar number of eggs and offspring during the first gonotrophic cycle, the survival rates of the F<jats:sub>1<\/jats:sub> generations were significantly lower as compared to the control group, suggesting transgenerational sublethal effects on the F<jats:sub>1<\/jats:sub> generation. Notably, surviving adult females had higher DENV\u20102 viral loads than the control group after spinetoram sublethal exposure. Mechanistically, transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult <jats:italic>Ae. aegypti<\/jats:italic>. In Aag2 cells, significant accumulation of apoptosis, mitochondrial reactive oxygen species production, and DENV\u20102 replication by spinetoram exposure consistently support our conclusion. Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito\u2010borne viruses.<\/jats:p>","DOI":"10.1111\/1744-7917.13116","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T09:50:07Z","timestamp":1662544207000},"page":"486-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sublethal exposure to spinetoram impacts life history traits and dengue virus replication in <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"Luoluo","family":"Wang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences South China Normal University  Guangzhou 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