{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:07:50Z","timestamp":1790680070305,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Asian citrus psyllid, <jats:italic>Diaphorina citri<\/jats:italic> Kuwayama, is among the most important pests of citrus. It is the main vector of the Huanglongbing (HLB) pathogen <jats:italic>Candidatus<\/jats:italic> Liberibacter asiaticus (<jats:italic>C<\/jats:italic>Las), which causes severe losses in citrus crops. Control of <jats:italic>D. citri<\/jats:italic> is therefore of paramount importance to reduce the spread of HLB. In this regard, using RNA interference (RNAi) to silence target genes is a useful strategy to control psyllids. In this study, using RNAi, we examined the biological functions of the <jats:italic>V\u2010ATPase subunit E<\/jats:italic> (<jats:italic>V\u2010ATP\u2010E<\/jats:italic>) gene of <jats:italic>D. citri<\/jats:italic>, including its effect on acquisition of <jats:italic>C<\/jats:italic>Las. The amino acid sequence of <jats:italic>V\u2010ATP\u2010E<\/jats:italic> from <jats:italic>D. citri<\/jats:italic> had high homology with proteins from other insects. <jats:italic>V\u2010ATP\u2010E<\/jats:italic> was expressed at all <jats:italic>D. citri<\/jats:italic> life stages analyzed, and the expression level in mature adults was higher than that of teneral adults. Silencing of <jats:italic>V\u2010ATP\u2010E<\/jats:italic> resulted in a significant increase in mortality, reduced body weight, and induced cell apoptosis of the <jats:italic>D. citri<\/jats:italic> midgut. The reduced expression of <jats:italic>V\u2010ATP\u2010E<\/jats:italic> was indicated to inhibit <jats:italic>C<\/jats:italic>Las passing through the midgut and into the hemolymph, leading to a majority of <jats:italic>C<\/jats:italic>Las being confined to the midgut. In addition, double\u2010stranded RNA of <jats:italic>D. citri V\u2010ATP\u2010E<\/jats:italic> was safe to non\u2010target parasitic wasps. These results suggest that <jats:italic>V\u2010ATP\u2010E<\/jats:italic> is an effective RNAi target that can be used in <jats:italic>D. citri<\/jats:italic> control to block <jats:italic>C<\/jats:italic>Las infection.<\/jats:p>","DOI":"10.1111\/1744-7917.13146","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T16:45:48Z","timestamp":1667925948000},"page":"1022-1034","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Silencing of <i>V\u2010ATPase\u2010E<\/i> gene causes midgut apoptosis of <i>Diaphorina citri<\/i> and affects its acquisition of Huanglongbing pathogen"],"prefix":"10.1111","volume":"30","author":[{"given":"Chang\u2010Fei","family":"Guo","sequence":"first","affiliation":[{"name":"Engineering Research Center of Biocontrol, Ministry of Education and Guangdong Province South China Agricultural University  Guangzhou China"},{"name":"Guangdong Laboratory for Lingnan 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