{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:30:59Z","timestamp":1793406659812,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their products. In sericulture, only the first filial generation (F<jats:sub>1<\/jats:sub>) hybrid eggs produced by cross\u2010breeding Japanese and Chinese original strains are usually used for the large\u2010scale breeding of silkworms, but this may result in uncontrolled transgene dispersal during the popularization and application of the F<jats:sub>1<\/jats:sub> hybrid transgenic eggs. To address this issue, we developed a safe and efficient strategy using the GAL4\/Upstream activating sequence (UAS) system, the FLP\/flippase recognition target (FRT) system, and the gonad\u2010specific expression gene promoters (<jats:italic>RSHP1p<\/jats:italic> and <jats:italic>Nanosp<\/jats:italic>) for the germ cell\u2010specific automatic excision of foreign DNA in the F<jats:sub>1<\/jats:sub> hybrid transgenic silkworms. We established 2 types of activator strains, R1p::GAL4\u2010Gr and Nsp::GAL4\u2010Gr, containing the testis\u2010specific <jats:italic>GAL4<\/jats:italic> gene expression cassettes driven by <jats:italic>RSHP1p<\/jats:italic> or <jats:italic>Nanosp<\/jats:italic>, respectively, and 1 type of effector strain, UAS::FLP\u2010Rg, containing the UAS\u2010linked <jats:italic>FLP<\/jats:italic> gene expression cassette. The FLP recombinase\u2010mediated sperm\u2010specific complete excision of <jats:italic>FRT<\/jats:italic>\u2010flanked target DNA in the F<jats:sub>1<\/jats:sub> double\u2010transgenic silkworms resulting from the hybridization of R1p::GAL4\u2010Gr and UAS::FLP\u2010Rg was 100%, whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4\u2010Gr and UAS::FLP\u2010Rg ranged from 13.73% to 80.3%. Additionally, we identified a gene, <jats:italic>sw11114<\/jats:italic>, that is expressed in both testis and ovary of <jats:italic>Bombyx mori<\/jats:italic>, and can be used to establish novel gonad\u2010specific expression systems in transgenic silkworms. This strategy has the potential to fundamentally solve the safety issue in the production of F<jats:sub>1<\/jats:sub> transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species.<\/jats:p>","DOI":"10.1111\/1744-7917.13219","type":"journal-article","created":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T12:44:10Z","timestamp":1687697050000},"page":"28-46","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An efficient and safe strategy for germ cell\u2010specific automatic excision of foreign DNA in F<sub>1<\/sub> hybrid transgenic silkworms"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2907-6901","authenticated-orcid":false,"given":"Dingpei","family":"Long","sequence":"first","affiliation":[{"name":"State Key Laboratory of Resource Insects Institute of Sericulture and Systems Biology, Southwest University  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