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In this study, we identified a testis\u2010specific gene <jats:italic>CG3526<\/jats:italic> in <jats:italic>Drosophila melanogaster<\/jats:italic>. Bioinformatics analysis showed that CG3526 contains a zinc binding domain and 2 C<jats:sub>2<\/jats:sub>H<jats:sub>2<\/jats:sub> type zinc fingers, and it is clustered to the vertebrate really interesting new gene (RING) family E3 ubiquitin\u2010protein ligases. When <jats:italic>CG3526<\/jats:italic> was knocked down by RNA interference (RNAi), the testis became much smaller in size, and the apical tip exhibited a sharp and thin end instead of the blunt and round shape in the control testis. More importantly, compared to the control flies, only a few mature sperm were present in the seminal vesicle of <jats:italic>C587\u2010Gal4\u00a0&gt;\u00a0CG3526<\/jats:italic> RNAi flies. Immunofluorescence staining of the testis from <jats:italic>CG3526<\/jats:italic> RNAi flies showed that the homeostasis of testis stem cell niche was disrupted, cell distribution in the apical tip was scattered, and the process of spermatogenesis was not completed. Furthermore, we found that the phenotype of <jats:italic>CG3526<\/jats:italic> RNAi flies\u2019 testis was similar to that of testis of Stat92E RNAi flies, the expression level of <jats:italic>CG3526<\/jats:italic> was significantly downregulated in the <jats:italic>Stat92E<jats:sup>F06346<\/jats:sup><\/jats:italic> mutant flies, and the promoter activity of <jats:italic>CG3526<\/jats:italic> was upregulated by STAT92E. Taken together, our results indicated that <jats:italic>CG3526<\/jats:italic> is a downstream effector gene in the JAK\u2010STAT signaling pathway that plays a key role in the spermatogenesis of <jats:italic>Drosophila<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1744-7917.13243","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T07:11:18Z","timestamp":1689750678000},"page":"79-90","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification and functional analysis of CG3526 in spermatogenesis of <i>Drosophila melanogaster<\/i>"],"prefix":"10.1111","volume":"31","author":[{"given":"Qihao","family":"Hu","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology School of Life 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