{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:50:25Z","timestamp":1790934625007,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Successful completion of spermatogenesis is crucial for the perpetuation of the species. In\n                    <jats:italic>Drosophila<\/jats:italic>\n                    , spermatid individualization, a process involving changes in mitochondrial structure and function is critical to produce functional mature sperm.\n                    <jats:italic>Ant2<\/jats:italic>\n                    , encoding a mitochondrial adenine nucleotide translocase, is highly expressed in male testes and plays a role in energy metabolism in the mitochondria. However, its molecular function remains unclear. Here, we identified an important role of\n                    <jats:italic>Ant2<\/jats:italic>\n                    in spermatid individualization. In\n                    <jats:italic>Ant2<\/jats:italic>\n                    knockdown testes, spermatid individualization complexes composed of F\u2010actin cones exhibited a diffuse distribution, and mature sperms were absent in the seminal vesicle, thus leading to male sterility. The most striking effects in\n                    <jats:italic>Ant2<\/jats:italic>\n                    \u2010knockdown spermatids were decrease in tubulin polyglycylation and disruption of proper mitochondria derivatives function. Excessive apoptotic cells were also observed in\n                    <jats:italic>Ant2<\/jats:italic>\n                    \u2010knockdown testes. To further investigate the phenotype of\n                    <jats:italic>Ant2<\/jats:italic>\n                    knockdown in testes at the molecular level, complementary transcriptome and proteome analyses were performed. At the mRNA level, 868 differentially expressed genes were identified, of which 229 genes were upregulated and 639 were downregulated induced via\n                    <jats:italic>Ant2<\/jats:italic>\n                    knockdown. iTRAQ\u2010labeling proteome analysis revealed 350 differentially expressed proteins, of which 117 proteins were upregulated and 233 were downregulated. The expression of glutathione transferase (GstD5, GstE5, GstE8, and GstD3), proteins involved in reproduction were significantly regulated at both the mRNA and protein levels. These results indicate that\n                    <jats:italic>Ant2<\/jats:italic>\n                    is crucial for spermatid maturation by affecting mitochondrial morphogenesis.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13309","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T09:05:02Z","timestamp":1702976702000},"page":"1055-1072","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Adenine nucleotide translocase 2<\/i>\n                    (\n                    <i>Ant2<\/i>\n                    ) is required for individualization of spermatogenesis of\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3045-8680","authenticated-orcid":false,"given":"Zhen","family":"He","sequence":"first","affiliation":[{"name":"Industrial Crops Institute Hubei Academy of Agriculture 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