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The identification of Y\u2010chromosome\u2010specific genes not only provides a theoretical basis for the study of male reproductive development, but also offers genetic control targets for agricultural pests. However, Y\u2010chromosome genes are rarely characterized due to their high repeatability and high heterochromatinization, especially in the oriental fruit fly. In this study, 1\u00a0011 Y\u2010chromosome\u2010specific candidate sequences were screened from 2 to 4\u00a0h <jats:italic>Bactrocera dorsalis<\/jats:italic> embryo datasets with the chromosome quotient method, 6 of which were identified as Y\u2010chromosome\u2010specific sequences by polymerase chain reaction, including <jats:italic>typo\u2010gyf<\/jats:italic>, a 19\u00a0126\u2010bp DNA sequence containing a 575\u2010amino acid open reading frame. Testicular deformation and a significant reduction in sperm number were observed after <jats:italic>typo\u2010gyf<\/jats:italic> knockdown with RNA interference in embryos. After <jats:italic>typo\u2010gyf<\/jats:italic> knockout with clustered regularly interspaced palindromic repeats (CRISPR) \/ CRISPR\u2010associated protein 9 in the embryonic stage, the sex ratio of the emergent adults was unbalanced, with far more females than males. A genotype analysis of these females with the Y\u2010chromosome gene <jats:italic>MoY<\/jats:italic> revealed no sex reversal. <jats:italic>Typo\u2010gyf<\/jats:italic> knockout led to the death of XY individuals in the embryonic stage. We conclude that <jats:italic>typo\u2010gyf<\/jats:italic> is an essential gene for male survival, and is also involved in testicular development and spermatogenesis. The identification of <jats:italic>typo\u2010gyf<\/jats:italic> and its functional verification provide insight into the roles of Y\u2010chromosome genes in male development.<\/jats:p>","DOI":"10.1111\/1744-7917.13311","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T10:11:50Z","timestamp":1704708710000},"page":"1270-1284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification and functional verification of Y\u2010chromosome\u2010specific gene <i>typo\u2010gyf<\/i> in <i>Bactrocera dorsalis<\/i>"],"prefix":"10.1111","volume":"31","author":[{"given":"Peipei","family":"Liu","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Centre for Horticultural and Urban Pests, Institute of Urban and Horticultural 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