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However, the molecular mechanisms involved in SLCMV transmission by\n                    <jats:italic>B.\u00a0tabaci<\/jats:italic>\n                    have yet to be understood. In this study, we identified an aminopeptidase N\u2010like protein (BtAPN) in\n                    <jats:italic>B.\u00a0tabaci<\/jats:italic>\n                    Asia\u00a0II\u00a01, an efficient vector of SLCMV, which is involved in the SLCMV transmission process. Through the use of glutathione S\u2010transferase pull\u2010down assay and LC\u2010MS\/MS analysis, we demonstrated the interaction between BtAPN and the coat protein (CP) of SLCMV. This interaction was further confirmed\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and we observed an induction of\n                    <jats:italic>BtAPN<\/jats:italic>\n                    gene expression following SLCMV infection. By interfering with the function of BtAPN, the quantities of SLCMV were significantly reduced in various parts of\n                    <jats:italic>B.\u00a0tabaci<\/jats:italic>\n                    Asia\u00a0II\u00a01, including the whole body, midgut, hemolymph, and primary salivary gland. Furthermore, we discovered that BtAPN is conserved in\n                    <jats:italic>B.\u00a0tabaci<\/jats:italic>\n                    Middle East\u2010Asia Minor 1 (MEAM1) and interacts with the CP of tomato yellow leaf curl virus (TYLCV), a begomovirus known to cause severe damage to tomato production. Blocking BtAPN with antibody led to a significant reduction in the quantities of TYLCV in whitefly whole body and organs\/tissues. These results demonstrate that BtAPN plays a generic role in interacting with the CP of begomoviruses and positively regulates their acquisition by the whitefly.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13336","type":"journal-article","created":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T21:14:00Z","timestamp":1708290840000},"page":"707-719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Role of aminopeptidase N\u2010like in the acquisition of begomoviruses by\n                    <i>Bemisia tabaci<\/i>\n                    , the whitefly vector"],"prefix":"10.1111","volume":"31","author":[{"given":"Yun\u2010Yun","family":"Fan","sequence":"first","affiliation":[{"name":"Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University  Hangzhou 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4702-3237","authenticated-orcid":false,"given":"Wilmer J.","family":"Cuellar","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Cassava Program International Center for Tropical Agriculture (CIAT)  Palmira Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7472-3944","authenticated-orcid":false,"given":"Xiao\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences Zhejiang University  Hangzhou 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