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Recent studies show that pesticide resistance can also be influenced by symbionts, but their involvement in this process in spider mites remains uncertain. Here, we found that infection with\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    , a well\u2010known bacterial reproductive manipulator, significantly increased mite survival after exposure to the insecticides abamectin, cyflumetofen, and pyridaben.\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    \u2010infected (WI) mites showed higher expression of detoxification genes such as P450, glutathione\u2010S\u2010transferase (GST), ABC transporters, and carboxyl\/cholinesterases. RNA interference experiments confirmed the role of the two above\u2010mentioned detoxification genes,\n                    <jats:italic>TuCYP392D2<\/jats:italic>\n                    and\n                    <jats:italic>TuGSTd05<\/jats:italic>\n                    , in pesticide resistance. Increased GST activities were also observed in abamectin\u2010treated WI mites. In addition, when wild populations were treated with abamectin, WI mites generally showed better survival than uninfected mites. However, genetically homogeneous mites with different\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strains showed similar survival. Finally, abamectin treatment increased\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    abundance without altering the mite's bacterial community. This finding highlights the role of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    in orchestrating pesticide resistance by modulating host detoxification. By unraveling the intricate interplay between symbionts and pesticide resistance, our study lays the groundwork for pioneering strategies to combat agricultural pests.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13341","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T22:06:30Z","timestamp":1708639590000},"page":"1822-1837","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The symbiont\n                    <i>Wolbachia<\/i>\n                    alleviates pesticide susceptibility in the two\u2010spotted spider mite\n                    <i>Tetranychus urticae<\/i>\n                    through enhanced host detoxification pathways"],"prefix":"10.1111","volume":"31","author":[{"given":"Qing\u2010Tong","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Entomology Nanjing Agricultural University  Nanjing 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