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Aquaporin channels are found in all life forms, where they facilitate environmental adaptation by allowing rapid trans\u2010cellular movement of water (classical aquaporins) or water and solutes such as glycerol (aquaglyceroporins). Here, we identified and characterized 2 aquaporin (AQP) homologs in <jats:italic>An. sinensis<\/jats:italic>: AsAQP2 (<jats:italic>An. sinensis<\/jats:italic> aquaglyceroporin) and AsAQP4 (<jats:italic>An. sinensis<\/jats:italic> aquaporin). When expressed in frog (<jats:italic>Xenopus laevis<\/jats:italic>) oocytes, AsAQP2 transported water, glycerol, and urea; AsAQP4 transported only water. Water permeation through AsAQP2 and AsAQP4 was inhibited by mercuric chloride. <jats:italic>AsAQP2<\/jats:italic> expression was slightly higher in adult female mosquitoes than in males, and <jats:italic>AsAQP4<\/jats:italic> expression was significantly higher in adult males. The 2 AsAQPs were highly expressed in Malpighian tubules and midgut. <jats:italic>AsAQP2<\/jats:italic> and <jats:italic>AsAQP4<\/jats:italic> expression was up\u2010regulated by blood feeding compared with sugar feeding. At freezing point (0\u00a0\u00b0C), the <jats:italic>AsAQP4<\/jats:italic> expression level increased and <jats:italic>An. sinensis<\/jats:italic> survival time reduced compared with those at normal temperature (26\u00a0\u00b0C). At low temperature (8\u00a0\u00b0C), the <jats:italic>AsAQP2<\/jats:italic> and <jats:italic>AsAQP4<\/jats:italic> expression levels decreased and survival time was significantly longer compared with those at 26\u00a0\u00b0C. These results suggest that AsAQP2 and AsAQP4 have roles in water homeostasis during blood digestion and in low temperature adaptation of <jats:italic>A. sinensis<\/jats:italic>. Together, our results show that the 2 AQPs are important for mosquito diuresis after blood feeding and when exposed to low temperatures.<\/jats:p>","DOI":"10.1111\/1744-7917.13359","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T08:58:39Z","timestamp":1711011519000},"page":"1743-1755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An aquaporin and an aquaglyceroporin have roles in low temperature adaptation of mosquitoes (<i>Anopheles sinensis<\/i>)"],"prefix":"10.1111","volume":"31","author":[{"given":"Yong\u2010Qiao","family":"Zhao","sequence":"first","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key laboratory Jiangsu Institute of Parasitic Diseases  Wuxi Jiangsu China"},{"name":"School of Public Health 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