{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:47:19Z","timestamp":1790948839290,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T00:00:00Z","timestamp":1713657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300389"],"award-info":[{"award-number":["32300389"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Spermatogenesis is critical for insect reproduction and the process is regulated by multiple genes. Glycosyltransferases have been shown to participate in the development of\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    ; however, their role in spermatogenesis is still unclear. In this study, we found that\n                    <jats:italic>\u03b1<\/jats:italic>\n                    1,4\u2010galactosyltransferase\u00a01 (\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    ) was expressed at a significantly higher level in the testis than in the ovary of\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . Importantly, the hatching rate was significantly decreased when\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    RNA interference (RNAi) males were crossed with\n                    <jats:italic>\n                      w\n                      <jats:sup>1118<\/jats:sup>\n                    <\/jats:italic>\n                    females, with only a few mature sperm being present in the seminal vesicle of\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    RNAi flies. Immunofluorescence staining further revealed that the individualization complex (IC) in the testes from\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    RNAi flies was scattered and did not move synchronically, compared with the clustered IC observed in the control flies. Terminal deoxyribonucleotide transferase (TdT)\u2010mediated dUTP nick end labeling (TUNEL) assay showed that apoptosis signals in the sperm bundles of\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    RNAi flies were significantly increased. Moreover, the expression of several individualization\u2010related genes, such as\n                    <jats:italic>Shrub<\/jats:italic>\n                    ,\n                    <jats:italic>Obp44a<\/jats:italic>\n                    and\n                    <jats:italic>Hanabi<\/jats:italic>\n                    , was significantly decreased, whereas the expression of several apoptosis\u2010related genes, including\n                    <jats:italic>Dronc<\/jats:italic>\n                    and\n                    <jats:italic>Drice<\/jats:italic>\n                    , was significantly increased in the testes of\n                    <jats:italic>\u03b14GT1<\/jats:italic>\n                    RNAi flies. Together, these results suggest that\n                    <jats:italic>\u03b1<\/jats:italic>\n                    4GT1 may play dual roles in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    spermatogenesis by regulating the sperm individualization process and maintaining the survival of sperm bundles.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13369","type":"journal-article","created":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T03:53:55Z","timestamp":1713671635000},"page":"127-139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dual roles of\n                    <i>\u03b1<\/i>\n                    1,4\u2010galactosyltransferase\u00a01 in spermatogenesis of\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1111","volume":"32","author":[{"given":"Yanhong","family":"Xiao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences South China Normal University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibo","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences South China Normal University  Guangzhou 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