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Obtaining more information about their relationship is crucial for a comprehensive understanding of tick and pathogen biology, pathogen transmission dynamics, and potential control strategies. RNA sequencing of uninfected and <jats:italic>B. microti<\/jats:italic>\u2010infected ticks resulted in the identification of 15\u00a0056 unigenes. Among these, 1\u00a0051 were found to be differentially expressed, with 796 being upregulated and 255 downregulated (<jats:italic>P<\/jats:italic> &lt; 0.05). Integrated transcriptomics datasets revealed the pivotal role of immune\u2010related pathways, including the Toll, Janus kinase\/signal transducer and activator of transcription (JAK\u2010STAT), immunodeficiency, and RNA interference (RNAi) pathways, in response to infection. Consequently, 3 genes encoding critical transcriptional factor Dorsal, Relish, and STAT were selected for RNAi experiments. The knockdown of Dorsal, Relish, and STAT resulted in a substantial increase in <jats:italic>Babesia<\/jats:italic> infection levels compared to the respective controls. These findings significantly advanced our understanding of tick\u2013<jats:italic>Babesia<\/jats:italic> molecular interactions and proposed novel tick antigens as potential vaccine targets against tick infestations and pathogen transmission.<\/jats:p>","DOI":"10.1111\/1744-7917.13384","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T04:39:21Z","timestamp":1717562361000},"page":"457-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptome study reveals tick immune genes restrict <i>Babesia microti<\/i> infection"],"prefix":"10.1111","volume":"32","author":[{"given":"Tingting","family":"Feng","sequence":"first","affiliation":[{"name":"Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, MOE Key Laboratory of Geriatric Diseases and Immunology Soochow University  Suzhou Jiangsu Province China"},{"name":"Central Laboratory The Fourth 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