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However, the biological functions of <jats:italic>ABHD5<\/jats:italic> in insects are poorly understood. In the current study, we describe the identification and characterization of the <jats:italic>ABHD5<\/jats:italic> gene in the lepidopteran model insect, <jats:italic>Bombyx mori<\/jats:italic>. The tissue expression profile investigated using quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR) reveals that <jats:italic>BmABHD5<\/jats:italic> is widely expressed in all tissues, with particularly high levels found in the midgut and testis. A binary transgenic CRISPR\/Cas9 system was employed to conduct a functional analysis of <jats:italic>BmABHD5<\/jats:italic>, with the mutation of <jats:italic>BmABHD5<\/jats:italic> leading to the dysregulation of lipid metabolism and excessive lipid accumulation in the larval midgut. Histological and physiological analysis further reveals a significant accumulation of lipid droplets in the midgut of mutant larvae. RNA\u2010seq and RT\u2010qPCR analysis showed that genes related to metabolic pathways were significantly affected by the absence of <jats:italic>BmABHD5<\/jats:italic>. Altogether, our data prove that <jats:italic>BmABHD5<\/jats:italic> plays an important role in regulating tissue\u2010specific lipid metabolism in the silkworm midgut.<\/jats:p>","DOI":"10.1111\/1744-7917.13386","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T08:48:59Z","timestamp":1717663739000},"page":"425-436","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>ABHD5<\/i> regulates midgut\u2010specific lipid homeostasis in <i>Bombyx mori<\/i>"],"prefix":"10.1111","volume":"32","author":[{"given":"Zhiping","family":"Xing","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology Jiangsu University of Science and Technology  Zhenjiang Jiangsu Province China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, The Sericultural Research Institute Chinese Academy 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