{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T04:09:22Z","timestamp":1747282162496,"version":"3.40.5"},"reference-count":109,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The escalating use of nanodiamonds (NDs) has raised concerns about their ecotoxicological impact, prompting exploration of therapeutic interventions. This paper pioneers the examination of Vitamin B12\u2010conjugated sericin (VB12\u2010SER) as a potential therapeutic approach against ND\u2010induced toxicity in darkling beetles (<jats:italic>Blaps polychresta<\/jats:italic>). The study analyzes mortality rates and organ\u2010specific effects, covering the testis, ovary, and midgut, before and after treatments. Following exposure to 10 mg NDs\/g body weight, within a subgroup of individuals termed ND2 with a mortality rate below 50%, two therapeutic treatments were administered, including pure sericin (SER) at 10 mg\/mL and VB12\u2010SER at 10.12 mg\/mL. Consequently, five experimental groups (control, SER, ND2, ND2+SER, ND2+SER+VB12) were considered. Kaplan\u2013Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30\u2010d period. Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs, including cell death, DNA damage, enzyme dysregulation, antioxidant imbalances, protein depletion, lipid peroxidation, and morphological deformities. In contrast, the proposed treatments, especially (ND2+SER+VB12), demonstrated remarkable recovery, highlighting VB12\u2010conjugated SER's potential in mitigating ND\u2010triggered adverse effects. Molecular docking simulations affirmed binding stability and favorable interactions of the VB12\u2010SER complex with target proteins. This research enhances understanding of NDs\u2019 effects on <jats:italic>B. polychresta<\/jats:italic>, proposing it as an effective bioindicator, and introduces VB12\u2010conjugated SER as a promising therapeutic strategy in nanotoxicological studies.<\/jats:p>","DOI":"10.1111\/1744-7917.13421","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T03:55:50Z","timestamp":1721188550000},"page":"551-584","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel treatment approach using vitamin B12\u2010conjugated sericin for mitigating nanodiamond\u2010induced toxicity in darkling beetles"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4467-7203","authenticated-orcid":false,"given":"Marwa","family":"Saad","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Zoology Alexandria University  Baghdad st., Qism Moharram Bek Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabila","family":"Selim","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Zoology Alexandria University  Baghdad st., Qism Moharram Bek Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-5742","authenticated-orcid":false,"given":"Lamia M.","family":"El\u2010Samad","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Zoology Alexandria University  Baghdad st., Qism Moharram Bek Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00319"},{"key":"e_1_2_9_3_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41936-018-0072-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2014.12.069"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2013.10.033"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167371"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141048"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2023.102101"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201504208"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-51254-1.00009-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaridenv.2018.04.008"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2206-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.064"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(81)90040-1"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.013441"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.38045\/ohrm.2020.1.10"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28596-y"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2019.09.003"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2022.115770"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2014.10.055"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2015.11.021"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241837"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.115661"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2010.10.115"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2022.110166"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.150644"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17712-z"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc02b02s25"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.130772"},{"key":"e_1_2_9_32_1","first-page":"114","volume-title":"Methods in Enzymology","author":"Floh\u00e9 L.","year":"1984"},{"key":"e_1_2_9_33_1","first-page":"453","article-title":"Malondialdehyde (MDA) as a lipid peroxidation marker","volume":"57","author":"Gawel S.","year":"2004","journal-title":"Wiadomosci Lekarskie (Warsaw, Poland: 1960)"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr500304f"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781482289343"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm-2018-0321"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c22804"},{"key":"e_1_2_9_38_1","doi-asserted-by":"crossref","unstructured":"Guo W. Yu J. Deng L. Chen Z. Chen Z. Liu H.et\u00a0al.(2018)Vitamin\u2010B12\u2010conjugated sericin micelles for targeting gastric cancer and reversing drug resistance. Available at SSRN 3212948:http:\/\/doi.org\/10.2139\/ssrn.3212948","DOI":"10.2139\/ssrn.3212948"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-022-01032-y"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl071521o"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06919"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102108"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2016.07.008"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.12703\/r\/10-13"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/fft2.44"},{"key":"e_1_2_9_46_1","first-page":"165","article-title":"Cell death: apoptosis versus necrosis (review)","volume":"21","author":"Kanduc D.","year":"2002","journal-title":"International Journal of Oncology"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0013873819070091"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2016.03.033"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2018.05.027"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2014.947704"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/su11102825"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.4001\/003.027.0418"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141743"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748233719893200"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.5.1415.bloodjournal8451415"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2007.12.001"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201703838"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11020482"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(85)90109-X"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201500304"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/21\/31\/315106"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/s18051494"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diamond.2021.108546"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0jm01570a"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-16073-9_6"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45228-9"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2011.209"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201300343"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b00839"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0013873817060045"},{"key":"e_1_2_9_72_1","unstructured":"National Center for Biotechnology Information(2023)PubChem Compound Summary for CID 71308682 CID 71308682.PubChem(accessed 20 October 2023).https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/71308682"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2023.112179"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-50703-9_1"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2019.105298"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12113380"},{"key":"e_1_2_9_78_1","first-page":"19","article-title":"Dynamics of the selected physiological responses in laboratory mice under the prolonged oral administration of nanodiamond suspentions","volume":"4","author":"Puzyr A.P.","year":"2004","journal-title":"Sibirskoe Medicinskoe Obozrenie"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1018"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.numecd.2005.05.003"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.126067"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744\u20107917.13357"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.4172\/2165-8064.1000390"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diamond.2007.07.020"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408430902831987"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp066387v"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-045037"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90265-0"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.51470\/PLANTARCHIVES.2022.v22.specialissue.036"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b02639"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2012.4952"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2014.10.003"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1208"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1071"},{"key":"e_1_2_9_95_1","first-page":"1","article-title":"Documenting tenebrionid diversity: progress on Blaps Fabricius (Coleoptera, Tenebrionidae, Tenebrioninae, Blaptini) systematics, with the description of five new species","volume":"282","author":"Soldati L.","year":"2017","journal-title":"European Journal of Taxonomy"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11040967"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.66.2715"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.21037\/arh.2018.12.02"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.5772\/65729"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.084"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-080-5_20"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(99)00011-1"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.109485"},{"key":"e_1_2_9_104_1","doi-asserted-by":"publisher","DOI":"10.18001\/TRS.7.5.1.201"},{"key":"e_1_2_9_105_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-3756-6"},{"key":"e_1_2_9_106_1","doi-asserted-by":"publisher","DOI":"10.1016\/0048-3575(82)90092-X"},{"key":"e_1_2_9_107_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940240103"},{"key":"e_1_2_9_108_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0567081"},{"key":"e_1_2_9_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diamond.2009.11.022"},{"key":"e_1_2_9_110_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.3627"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.13421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T10:20:38Z","timestamp":1747218038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.13421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,16]]},"references-count":109,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/1744-7917.13421"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.13421","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"type":"print","value":"1672-9609"},{"type":"electronic","value":"1744-7917"}],"subject":[],"published":{"date-parts":[[2024,7,16]]},"assertion":[{"value":"2024-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}