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However, large\u2010scale production of bioactive recombinant human CNTF protein remains to be explored. Herein, this study aims to express a bioactive human CNTF protein on a large scale by genetically engineering a silk gland bioreactor of silkworm. Our results showed that CNTF protein was successfully expressed in the middle silk gland (MSG) of silkworm, which can be secreted into the silks with the amount of 3.2\u00a0mg\/g cocoons. The fabrication of human CNTF\u2010functionalized silk material was able to promote proliferation and migration of neural cells when compared to the natural silk protein. Importantly, this functional silk material could also facilitate neurite outgrowth of mouse retinal ganglion cell (RGC\u20105) cells. All these data demonstrated a high bioactivity of the recombinant human CNTF protein expressed in the MSG of silkworm. The further fabrication of different silk materials with CNTF bioactivity will give biomedical applications in tissue engineering and neuroregeneration.<\/jats:p>","DOI":"10.1111\/1744-7917.13442","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T02:35:45Z","timestamp":1725244545000},"page":"809-820","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transgenic silkworm expressing bioactive human ciliary neurotrophic factor for biomedical application"],"prefix":"10.1111","volume":"32","author":[{"given":"Weiqun","family":"Lan","sequence":"first","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City &amp; Southwest University, Biological Science Research Center Southwest University  Chongqing China"},{"name":"Key Laboratory for Germplasm Creation in Upper Reaches of the Yangtze River Ministry of Agriculture and Rural 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