{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:58:31Z","timestamp":1767898711637,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["No. 2021YFC2600400"],"award-info":[{"award-number":["No. 2021YFC2600400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the organisms with XX\/XY sex chromosomes, Y chromosome is unique to males and plays an important role in male reproductive development. The study of Y chromosome genes will contribute to the development of pest genetic prevention and control technology. In this study, we identified 9 Y chromosome genes in <jats:italic>Zeugodacus tau<\/jats:italic> (Diptera: Tephritidae), including gene <jats:italic>16222<\/jats:italic>. Protein structure analysis showed that 16222 was highly similar to odorant binding protein, and thus gene <jats:italic>16222<\/jats:italic> was named <jats:italic>obp\u2010Y<\/jats:italic>. <jats:italic>Obp\u2010Y<\/jats:italic> knockout (KO) significantly reduced hatching rate of offspring. Sperm detection results showed that <jats:italic>obp\u2010Y<\/jats:italic> KO did not affect sperm number in the testes or sperm transfer during mating. We further examined the storage of sperms in females, and found that sperms in females mating with wild\u2010type males began to transfer from spermathecal ducts to the spermathecae at hour 0 after the end of mating (AEM), and at 0\u201324\u00a0h AEM, the sperm count in the spermathecae gradually increased. However, no sperms were observed in spermathecae of females mating with mutant males at hours 0, 4, 8, 24 and 48 AEM. In summary, this study revealed that Y chromosome gene <jats:italic>obp\u2010Y<\/jats:italic> was necessary for the storage of sperms in females. Our findings not only provide theoretical basis for elucidating the function of the Y chromosome, but also offer a molecular target for the genetic control over <jats:italic>Z. tau<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1744-7917.13453","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:08:34Z","timestamp":1727741314000},"page":"1213-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A newly identified Y chromosome gene <i>obp\u2010Y<\/i> is required for sperm storage in female <i>Zeugodacus tau<\/i> (Diptera: Tephritidae)"],"prefix":"10.1111","volume":"32","author":[{"given":"Peipei","family":"Liu","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziniu","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imran","family":"Afzal","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Qiao","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Hubei Hongshan Laboratory, China\u2010Australia Joint Research Center for Horticultural and Urban Pests, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan Hubei 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