{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:56:26Z","timestamp":1770746186419,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872972"],"award-info":[{"award-number":["31872972"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170501"],"award-info":[{"award-number":["32170501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Insect lytic polysaccharide monooxygenases (LPMO15s) are newly discovered copper\u2010dependent enzymes that promote chitin degradation in insect through oxidative cleavage of glycosidic bonds. They are potential pesticide targets due to their critical role for chitin turnover in the integument, trachea, and peritrophic matrix of the midgut during insect molting. However, the knowledge about whether and how LPMO15s participate in chitin turnover in other tissues is still insufficient. Here, using the orthopteran pest <jats:italic>Locusta migratoria<\/jats:italic> as a model, a novel alternative splicing site of <jats:italic>LmLPMO15\u20101<\/jats:italic> was discovered and it produces 2 variants, <jats:italic>LmLPMO15\u20101a<\/jats:italic> and <jats:italic>LmLPMO15\u20101b<\/jats:italic>. The transcripts of <jats:italic>LmLPMO15\u20101a<\/jats:italic> and <jats:italic>LmLPMO15\u20101b<\/jats:italic> were specifically expressed in the trachea and foregut, respectively. RNA interference targeting <jats:italic>LmLPMO15\u20101<\/jats:italic> (a common fragment shared by both <jats:italic>LmLPMO15\u20101a<\/jats:italic> and <jats:italic>LmLPMO15\u20101b<\/jats:italic>), a specific region of <jats:italic>LmLPMO15\u20101a<\/jats:italic> or <jats:italic>LmLPMO15\u20101b<\/jats:italic> all significantly reduced survival rate of nymphs and induced lethal phenotypes with developmental stasis or molt failure. Ultrastructure analysis demonstrated that <jats:italic>LmLPMO15\u20101b<\/jats:italic> was specifically involved in foregut old cuticle degradation, while <jats:italic>LmLPMO15\u20101a<\/jats:italic> was exclusively responsible for the degradation of the tracheal old cuticle. This study revealed <jats:italic>LmLPMO15\u20101<\/jats:italic> achieved tissue\u2010specific functional differentiation through alternative splicing, and proved the significance of the spliced variants during insect growth and development. It provides new strategies for pest control targeting <jats:italic>LPMO15\u20101<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1744-7917.13469","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T02:09:14Z","timestamp":1731636554000},"page":"1241-1255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tissue\u2010specific alternative splicing and the functional differentiation of <i>LmLPMO15\u20101<\/i> in <i>Locusta migratoria<\/i>"],"prefix":"10.1111","volume":"32","author":[{"given":"Lin","family":"Kong","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology  Dalian Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiying","family":"Hu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology  Dalian Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology  Dalian Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0397-1630","authenticated-orcid":false,"given":"Mingbo","family":"Qu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology  Dalian Liaoning Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112422200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2009.02.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2003.11.004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c00888"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2020.107553"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2558"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001978"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00943"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2019.02.015"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2020.111316"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2020.09.013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(97)00078-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-023-01722-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.01.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-023-01697-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.09.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-023-01677-7"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00709"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384747-8.10007-8"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007307"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760170179"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03469-8"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12791"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2011.09.001"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2012.01145.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7917.2012.01573.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03142-x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj1342"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.14464"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20170549"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.01.006"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192231"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37488-5"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13072-020-00356-6"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.06.017"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13313"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.720581"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.08.001"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12874"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11121778"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2018.09.001"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7917.2012.01568.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5308"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010715-023933"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2007.06.011"}],"container-title":["Insect 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