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In the current study, a gene with repetitive sequence was identified within the silkworm genome. Designated as BmMuc91C, it contains a dozen repeated motifs \u201cPSSSYGAPX\u201d and \u201cGGYSSGGX\u201d in its sequence. <jats:italic>BmMuc91C<\/jats:italic> exhibits specific expression in the tracheal system of silkworm larvae, with significantly higher expression levels during the molting stage. Overexpression of <jats:italic>BmMuc91C<\/jats:italic> in individual silkworms resulted in a marked increase in tracheal diameter, particularly during the molting stage. Immunofluorescence staining using a BmMuc91C antibody revealed a noticeable thickening of the apical extracellular matrix in the trachea. Tensile testing confirmed a considerable enhancement in tracheal elasticity. Additionally, a <jats:italic>BmMuc91C<\/jats:italic> mutation strain of silkworms was generated using the clustered regularly interspaced short palindromic repeats (CRISPR) \/ CRISPR\u2010associated nuclease 9 system. Although no significant differences were observed in the growth, development, and molting of <jats:italic>BmMuc91C<\/jats:italic> mutant silkworms, mechanical tests demonstrated a decrease in tracheal elasticity. Transcriptomic techniques revealed that a significant number of cuticular and chitin\u2010binding proteins were among the differentially expressed genes between mutant and wild\u2010type silkworms. Furthermore, the recombined BmMuc91C protein was successfully expressed using the <jats:italic>Escherichia coli<\/jats:italic> system. Cross\u2010linking experiments with horseradish peroxidase demonstrated the formation of macromolecular complexes of BmMuc91C, which exhibited spontaneous luminescent properties under ultraviolet light. This research sheds light on the role of elastic proteins in insect tracheae and provides valuable insights for the development of elastic biomaterials.<\/jats:p>","DOI":"10.1111\/1744-7917.13492","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T12:40:38Z","timestamp":1737031238000},"page":"1269-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Elasticity of trachea in the silkworm: A role of gene <i>BmMuc91C<\/i>"],"prefix":"10.1111","volume":"32","author":[{"given":"Yifei","family":"Chen","sequence":"first","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center Southwest University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haonan","family":"Dong","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm 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