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This process is characterized by arrested ovarian development and lipid accumulation in females. A reduction in juvenile hormone (JH) biosynthesis is known to initiate reproductive diapause, but its regulatory mechanism remains unclear. Seven up (Svp), a transcription factor from the nuclear receptor family, plays a crucial role in various developmental processes in insects. In this study, using the cabbage beetle\n                    <jats:italic>Colaphellus bowringi<\/jats:italic>\n                    as a model, we observed higher expression of\n                    <jats:italic>Svp<\/jats:italic>\n                    in the heads of female adults under reproductive photoperiodic conditions (short\u2010day [SD]) compared to diapause conditions (long\u2010day [LD]). RNA interference\u2010mediated knockdown of\n                    <jats:italic>Svp<\/jats:italic>\n                    in SD females induced typical diapause phenotypes, including ovarian arrest and lipid accumulation. The application of methoprene (ME), a JH receptor agonist, reversed these diapause phenotypes and restored reproduction, indicating that\n                    <jats:italic>Svp<\/jats:italic>\n                    \u2019s regulation of reproductive diapause is dependent on JH signaling. Additionally,\n                    <jats:italic>Svp<\/jats:italic>\n                    knockdown led to the downregulation of JH pathway genes and a reduction in JH titers. Further evidence suggested that\n                    <jats:italic>Svp<\/jats:italic>\n                    regulates the expression of JHAMT1, a critical gene in JH biosynthesis, which determines diapause entry in\n                    <jats:italic>C. bowringi<\/jats:italic>\n                    . These findings suggest that diapause\u2010inducing photoperiods suppress\n                    <jats:italic>Svp<\/jats:italic>\n                    expression, blocking JH production and triggering diapause. This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production, providing a potential target for controlling pests capable of entering reproductive diapause.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13497","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T12:01:04Z","timestamp":1737028864000},"page":"85-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Seven up<\/i>\n                    regulates reproductive diapause initiation via juvenile hormone biosynthesis in the cabbage beetle\n                    <i>Colaphellus bowringi<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Kou","family":"Wang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Lian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Zi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4985-0618","authenticated-orcid":false,"given":"Wen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Alberta, G\u2010504, Biological Sciences Bldg.  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1718-1026","authenticated-orcid":false,"given":"Xiao\u2010Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40778-017-0070-z"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40234"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706865114"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.220031"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004425"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1990.tb01376.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/9781108609364"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5967.2008.00139.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.47.091201.145137"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384749-2.10010-X"},{"key":"e_1_2_7_12_1","unstructured":"Dewilde J. 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