{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:54:47Z","timestamp":1783464887607,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32220103009"],"award-info":[{"award-number":["32220103009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    RNA\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sup>6<\/jats:sup>\n                    \u2010methyladenine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) modification represents a pivotal epigenetic modification that facilitates the remodeling of gene expression and regulates a variety of biological processes via certain post\u2010transcriptional mechanisms. However, the specific function of RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in insect male reproduction remains unclear. In this study, we explored the molecular mechanism by which METTL3\/METTL14\u2010mediated RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A modification regulates male reproduction in the invasive pest\n                    <jats:italic>Bactrocera dorsalis<\/jats:italic>\n                    . The results showed that\n                    <jats:italic>BdMettl3<\/jats:italic>\n                    and\n                    <jats:italic>BdMettl14<\/jats:italic>\n                    were highly expressed in fat body (FB) and male accessory glands (MAGs). Knockout of\n                    <jats:italic>BdMettl3<\/jats:italic>\n                    or\n                    <jats:italic>BdMettl14<\/jats:italic>\n                    decreased the expression level of m\n                    <jats:sup>6<\/jats:sup>\n                    A in\n                    <jats:italic>B. dorsalis<\/jats:italic>\n                    , resulting in testicular deformities and a significant reduction of viable sperm number. Specifically,\n                    <jats:italic>BdMettl3<\/jats:italic>\n                    or\n                    <jats:italic>BdMettl14<\/jats:italic>\n                    knockout reduced the titer of 20\u2010hydroxyecdysone (20E, the active form of ecdysone) in males. The messenger RNA (mRNA) of\n                    <jats:italic>Disembodied<\/jats:italic>\n                    , one of the 20E synthesis genes, was modified by m\n                    <jats:sup>6<\/jats:sup>\n                    A, and its expression increased the titer of 20E. The mRNA m\n                    <jats:sup>6<\/jats:sup>\n                    A level of\n                    <jats:italic>Disembodied<\/jats:italic>\n                    obviously decreased after the knockout of\n                    <jats:italic>BdMettl3<\/jats:italic>\n                    or\n                    <jats:italic>BdMettl14<\/jats:italic>\n                    , suggesting that RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A modification regulates testis development and fecundity by modulating 20E synthesis. Taken together, this study indicates that METTL3\/METTL14\u2010mediated RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A modification presents a new regulatory mechanism for male reproduction in\n                    <jats:italic>B. dorsalis<\/jats:italic>\n                    , serving as a potential target for the control of\n                    <jats:italic>B. dorsalis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.13510","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T01:28:42Z","timestamp":1740014922000},"page":"71-84","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["METTL3\/METTL14\u2010mediated RNA m\n                    <sup>6<\/sup>\n                    A modification is involved in male reproductive development in\n                    <i>Bactrocera dorsalis<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Qiuyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziniu","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Qiao","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenjun","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6368-5903","authenticated-orcid":false,"given":"Hongyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.10.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32497-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-021-00837-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.17322"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.50.071803.130428"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11112"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-052521-035949"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202307650"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20577"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.07.061"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1759-7714.2011.00043.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20568"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2022.151916"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130607"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.07.016"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23094630"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1432"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13311"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620760114"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100616-060758"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04272-9"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2017.10.008"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12542"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.3"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809264105"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2021.02.003"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18117"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006788"},{"key":"e_1_2_7_31_1","article-title":"Ubiquitous knockdown of Mettl3 using TRiP.GL01126 results in spermatid mislocalization during Drosophila spermatogenesis","volume":"2022","author":"Rowe L.","year":"2022","journal-title":"Micropublication Biology"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-020117-043258"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.05.048"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.114304"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006788"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-11-76"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717794115"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108580"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12730"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2902"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.100"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe5903"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0040-8"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2101442118"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-00982-9"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180158"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.132"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21355"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.132"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43987-5"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.10.015"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.13510","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.13510","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.13510","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:08:16Z","timestamp":1771049296000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.13510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,19]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/1744-7917.13510"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.13510","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,19]]},"assertion":[{"value":"2024-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}