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However, the optimal efficiency and simplicity of delivering exogenous guide RNA\u2010clustered regularly interspaced short palindromic repeats\u2010associated protein 9 complexes into target tissues are crucial for successful gene editing. The Receptor\u2010Mediated Ovary Transduction of Cargo (ReMOT) strategy, which simplifies the delivery process, target\u2010site selection, technical requirements, and delivery cost compared with embryonic microinjection, enabling efficient editing at the germline level, is gaining increasing attention. Although the feasibility and advantages of this technique have been demonstrated in various insect species, further optimization of operational details and the overcoming of further bottlenecks are still required. This review focuses on advances in developing ReMOT as a valuable technology, exploring its applicability, rationale for selecting the ovary as a delivery target site, factors influencing its efficiency, and improvement recommendations. The versatility and effectiveness of ReMOT make it a promising method for researchers looking to make precise genetic modifications with greater ease and efficiency.<\/jats:p>","DOI":"10.1111\/1744-7917.70003","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T01:48:51Z","timestamp":1740016131000},"page":"2-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Increased versatility and convenience: Advances and strategy optimization of Receptor\u2010Mediated Ovary Transduction of Cargo\u2010mediated genetic modification in insects"],"prefix":"10.1111","volume":"33","author":[{"given":"Xia","family":"Ling","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Vector Control and Utilization, Institute of Entomology and Molecular Biology, College of Life Sciences Chongqing Normal University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Vector Control and Utilization, Institute of Entomology and Molecular Biology, College of Life Sciences Chongqing Normal University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Feng","family":"Hong","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Vector Control and Utilization, Institute of Entomology and Molecular Biology, College of Life Sciences Chongqing Normal University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Ping","family":"Jiang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Vector Control and Utilization, Institute of Entomology and Molecular Biology, College of Life Sciences Chongqing Normal University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Sun","sequence":"additional","affiliation":[{"name":"Xunjian Life Science &amp; Technology Co. 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