{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:51:43Z","timestamp":1783464703437,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202405"],"award-info":[{"award-number":["32202405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1400800"],"award-info":[{"award-number":["2023YFD1400800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011229","name":"State Key Laboratory of Integrated Pest Management","doi-asserted-by":"publisher","award":["IPM2001"],"award-info":[{"award-number":["IPM2001"]}],"id":[{"id":"10.13039\/501100011229","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Spodoptera frugiperda<\/jats:italic>\n                    (Lepidoptera: Noctuidae) is a serious invasive pest, which has attracted concern regarding the effectiveness of environmental bioinsecticide as a substitution for synthetic insecticide in controlling its damage to numerous agricultural crops in recent years. Hence, laboratory and field experiments have investigated insecticidal toxicity in\n                    <jats:italic>S. frugiperda<\/jats:italic>\n                    by 5 insecticides and have determined the activity of acetylcholinesterase (AChE) and detoxifying enzymes, namely mixed function oxidase (MFO), carboxylesterase (CarE), and glutathione S\u2010transferase (GST) on 3rd instar larvae at 25% lethal concentration (LC\n                    <jats:sub>25<\/jats:sub>\n                    ) to explore detoxification mechanisms. The results showed that the most effective insecticides were 3.0% emamectin benzoate (0.024 mg\/L), 60 g\/L spinetoram (0.282 mg\/L), and 1.3% matrine (0.380 mg\/L) at 48 h depending on LC\n                    <jats:sub>50<\/jats:sub>\n                    values. The field efficiencies of emamectin benzoate and spinetoram were over 80% at 24 h and 90% at 72 h, indicating that both insecticides had acute and long\u2010lasting toxicity on\n                    <jats:italic>S. frugiperda<\/jats:italic>\n                    ; matrine had extended\u2010release toxicity with 90% field efficiency at 72 h. Emamectin benzoate and spinetoram activated the activities of AChE, MFO and GST, and spinetoram decreased in CarE activity; cyantraniliprole induced an increase of 4 detoxifying enzymes; spinosad increased the activities of AChE, MFO, and CarE but not GST. Furthermore, matrine had an inhibiting effect on AChE and acceleration on CarE and GST. Overall results obviously depicted that semi\u2010synthetic insecticide spinetoram and bioinsecticide matrine were recommended to control\n                    <jats:italic>S. frugiperda<\/jats:italic>\n                    with effective and long\u2010lasting toxicity. Moreover, this study will provide basic information for sustainable control of\n                    <jats:italic>S. frugiperda<\/jats:italic>\n                    under field conditions in China.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70005","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:15:06Z","timestamp":1742170506000},"page":"1055-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bioinsecticide control and enzymatic responses in\n                    <i>Spodoptera frugiperda<\/i>"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7044-6353","authenticated-orcid":false,"given":"Zhuo","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Yan","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Rui","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"},{"name":"College of Plant Protection Shandong Agricultural University  Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan\u2010Lin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Public Health Jinan Vocational College of Nursing  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Yuan","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Public Health Jinan Vocational College of Nursing  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Long","family":"Xing","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"},{"name":"College of Plant Protection Shandong Agricultural University  Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Cheng","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute of Plant Protection Shandong Academy of Agricultural Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1","article-title":"Evaluation of the attack rates and level of damages by the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), affecting corn\u2010crops in the northeast of Argentina","volume":"45","author":"Ayala O.R.","year":"2013","journal-title":"Revista de la Facultad de Ciencias Agrarias"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jen.12565"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/60.6.1215"},{"key":"e_1_2_8_5_1","first-page":"153","article-title":"Residue and decline dynamics of spinetoram in paddy water, soil, and rice straw","volume":"16","author":"Chen G.","year":"2014","journal-title":"China Journal of Pesticide Science"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1180655"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744\u20107917.13376"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph8051402"},{"key":"e_1_2_8_9_1","first-page":"576","article-title":"The environment behavior of matrine in the soil","volume":"19","author":"Dou X.M.","year":"2017","journal-title":"China Journal of Pesticide Science"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics11030215"},{"key":"e_1_2_8_11_1","first-page":"558","article-title":"Toxicity of spinetoram and its effects on the detoxifying enzyme and acetyl cholinesterase activities in Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae","volume":"63","author":"Gao Z.P.","year":"2020","journal-title":"Acta Entomologica Sinica"},{"key":"e_1_2_8_12_1","first-page":"27","article-title":"Field efficacy trials of different bioinsecticide against rice leaf roller larvae","volume":"6","author":"Guo R.G.","year":"2021","journal-title":"Hubei Plant Protection"},{"key":"e_1_2_8_13_1","first-page":"880","article-title":"Detection of insecticide susceptibility and target\u2010site mutations in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)","volume":"12","author":"Guo Z.M.","year":"2022","journal-title":"International Journal of Pest Management"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c04189"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/tov021"},{"key":"e_1_2_8_16_1","first-page":"86","article-title":"The effects of three biopesticides on the indoor toxicity and detoxification enzyme activity of the sycamore square winged web bug","volume":"48","author":"Hao D.J.","year":"2012","journal-title":"Forestry Science"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75053-1"},{"key":"e_1_2_8_18_1","first-page":"477","article-title":"Toxicity test of botanical insecticide matrine to Bombyx mori","volume":"41","author":"Huang L.","year":"2015","journal-title":"Science of Sericulture"},{"key":"e_1_2_8_19_1","first-page":"158","article-title":"Effects of four plant\u2010derived compounds on the survival rate and activities of detoxification enzymes and protective enzymes in the grasshopper Oedaleus asiaticu","volume":"48","author":"Huang X.B.","year":"2021","journal-title":"Journal of Plant Protection"},{"key":"e_1_2_8_20_1","first-page":"101","article-title":"Determination of indoor toxicity of six insecticides to the larvae of the corn meadow armyworm","volume":"28","author":"Jiang Y.J.","year":"2022","journal-title":"Anhui Agricultural Bulletin"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2021.101175"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2022\/1659"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2018.08.047"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects12080758"},{"key":"e_1_2_8_25_1","first-page":"559","article-title":"Response comparison of protective and detoxification enzymes in Spodoptera frugiperda (J. E. Smith) and Spodoptera litura (Fabricius) larvae to two insecticides","volume":"52","author":"Li H.","year":"2021","journal-title":"Journal of Southern Agricultural"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-011-0404-y"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105685"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.15811"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.5138"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13190"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13348"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-83582010000100013"},{"key":"e_1_2_8_33_1","first-page":"69","article-title":"Potential geographic distribution of Spodoptera frugiperda in China based on MaxEnt model","volume":"33","author":"Lin W.","year":"2019","journal-title":"Plant Quarantine"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-022-01004-3"},{"key":"e_1_2_8_35_1","first-page":"564","article-title":"Gustatory electrophysiological and behavioral responses of Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae to three plant secondary metabolite","volume":"60","author":"Ma Y.","year":"2023","journal-title":"Chinese Journal of Applied Entomology"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111764"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4001\/003.026.0286"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15090705"},{"key":"e_1_2_8_39_1","first-page":"412","article-title":"Laboratory toxicity of seven insecticides against Spodoptera frugiperda larva in Jiangxi province","volume":"46","author":"Qi X.W.","year":"2023","journal-title":"Biological Disaster Science"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.18474\/0749-8004-47.2.177"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18243"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.11.014"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.113802"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000748532300038X"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1794"},{"key":"e_1_2_8_46_1","first-page":"394","article-title":"Research status and prospects of emamectin benzoate","volume":"54","author":"Wang L.L.","year":"2015","journal-title":"Agrochemistry"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toac059"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13142"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.106000"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12940"},{"key":"e_1_2_8_51_1","first-page":"464","article-title":"Effects of sublethal concentration of chlorpyrifos on insecticide sensitivity and detoxification enzyme activity of diamondback moth","volume":"13","author":"Xing J.","year":"2011","journal-title":"Journal of Pesticide Science"},{"key":"e_1_2_8_52_1","first-page":"65","article-title":"Spinosad induces programmed cell death involves mitochondrial dysfunction and cytochrome C release in Spodoptera frugiperda Sf9 cells","volume":"11","author":"Yang M.","year":"2017","journal-title":"Chemosphere"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12903"},{"key":"e_1_2_8_54_1","first-page":"19","article-title":"Effect of matrine pesticide on detoxifying enzyme activities of tussah larvae before and after poisoning","volume":"45","author":"Yang S.F.","year":"2024","journal-title":"North Sericulture"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.820778"},{"key":"e_1_2_8_56_1","first-page":"634","article-title":"Effects of sublethal doses of cyantraniliprole on the growth and development and the activities of detoxifying enzymes in Spodoptera exigua (Lepidoptera: Noctuidae)","volume":"58","author":"Yu H.L.","year":"2015","journal-title":"Acta Entomologica Sinica"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(20)63392-5"},{"key":"e_1_2_8_58_1","first-page":"10","article-title":"Laboratory test on the control efficacy of common chemical insecticides against Spodoptera frugiperda","volume":"45","author":"Zhao S.Y.","year":"2019","journal-title":"Journal of Plant Protection"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.104623"},{"key":"e_1_2_8_60_1","first-page":"89","article-title":"Effects of chlorantraniliprole on activities of detoxification enzymes and growth and reproduction of Ostrinia furnacalis","volume":"44","author":"Zhi H.Y.","year":"2021","journal-title":"Journal of Nanjing Agricultural University"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.70005","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:14:29Z","timestamp":1781331269000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.70005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,16]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/1744-7917.70005"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.70005","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,16]]},"assertion":[{"value":"2024-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}