{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:56:02Z","timestamp":1781333762465,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Leptinotarsa decemlineata<\/jats:italic>\n                    (Say) (Colorado potato beetle, CPB) is a notorious pest of potatoes. Its hardened forewings, known as elytra, protect the hindwings and the abdomen against enemies and chemical pesticides. Ubx plays a crucial role in differentiating insect wings, but the molecular mechanism remains unclear. Our research showed that\n                    <jats:italic>Ubx<\/jats:italic>\n                    is highly expressed in the T3 thoracic segment (T3) and appendages, while\n                    <jats:italic>Ubx<\/jats:italic>\n                    is absent in the forewings. RNA interference for\n                    <jats:italic>Ubx<\/jats:italic>\n                    resulted in the dorsal plate of the T3 being shifted toward the T2, hindwings were sheathed from the membranous in adults, and forewings were curling. The hindwing development\u2010related genes downstream of\n                    <jats:italic>Ubx<\/jats:italic>\n                    , including\n                    <jats:italic>LdDpp<\/jats:italic>\n                    ,\n                    <jats:italic>LdIro<\/jats:italic>\n                    ,\n                    <jats:italic>LdVg<\/jats:italic>\n                    ,\n                    <jats:italic>LdWg<\/jats:italic>\n                    , and\n                    <jats:italic>LdASH<\/jats:italic>\n                    , were significantly up\u2010regulated in\n                    <jats:italic>Ubx<\/jats:italic>\n                    \u2010inhibited insects compared with the control, by 2.40, 1.88, 1.81, 1.89, and 2.81 times, respectively. After feeding 20\u2010hydrosyecdysone (20E), the forewing was flatter and more stretched than the control, leading to increased wing area, which indicates\n                    <jats:italic>Ubx<\/jats:italic>\n                    regulation is associated with the feedback of ecdysteroids. The expression levels of 3 ecdysteroid receptor genes\n                    <jats:italic>EcR<\/jats:italic>\n                    ,\n                    <jats:italic>EcRA<\/jats:italic>\n                    , and\n                    <jats:italic>EcRB<\/jats:italic>\n                    were significantly lower in knocking down\n                    <jats:italic>LdUbx<\/jats:italic>\n                    insects than the control and was able to be recovered by 20E feeding, suggesting that ecdysteroid is associated with\n                    <jats:italic>LdUbx<\/jats:italic>\n                    \u2010regulated wing development. Cosilencing\n                    <jats:italic>LdUbx<\/jats:italic>\n                    and\n                    <jats:italic>LdAkt<\/jats:italic>\n                    genes significantly inhibited the growth of forewings and hindwings, reducing the area of the wings. Together, our findings suggested that LdUbx regulates wing development along with ecdysteroid and insulin\u2010like peptide signaling pathways, which provides a novel molecular mechanism of wing development and differentiation in insects.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70008","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T22:11:42Z","timestamp":1741731102000},"page":"841-854","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Ultrabithorax<\/i>\n                    regulates\n                    <i>Leptinotarsa decemlineata<\/i>\n                    wing development along with insulin and\n                    <i>ecdysteroid<\/i>\n                    signaling pathways"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2273-682X","authenticated-orcid":false,"given":"An\u2010Di","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Agriculture Xinjiang Agricultural University Urumqi China"},{"name":"Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences Urumqi China"},{"name":"Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Urumqi  Xinjiang China"},{"name":"Xinjiang Key Laboratory of Agricultural Biosafety Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zun\u2010Zun","family":"Jia","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences Urumqi China"},{"name":"Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Urumqi  Xinjiang China"},{"name":"Xinjiang Key Laboratory of Agricultural Biosafety Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Wu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences Urumqi China"},{"name":"Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Urumqi  Xinjiang China"},{"name":"Xinjiang Key Laboratory of Agricultural Biosafety Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Hua","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences Urumqi China"},{"name":"Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Urumqi  Xinjiang China"},{"name":"Xinjiang Key Laboratory of Agricultural Biosafety Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tursun","family":"Ahmat","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences Urumqi China"},{"name":"Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Urumqi  Xinjiang China"},{"name":"Xinjiang Key Laboratory of Agricultural Biosafety Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010He","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Chinese Academy of Sciences Beijing 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