{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:24:43Z","timestamp":1790814283471,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272628"],"award-info":[{"award-number":["32272628"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    LIM\u2010HD (homeodomain) protein is essential for the specific differentiation of various cells and tissues due to its conserved HD. Within the LIM subclass, the LIM\u2010HD transcription factor 1 alpha (\n                    <jats:italic>Lmx1a<\/jats:italic>\n                    ) remains poorly characterized in insects. This study investigates the expression patterns of\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    in the 28\u2010spotted lady beetle,\n                    <jats:italic>Henosepilachna vigintioctopunctata<\/jats:italic>\n                    , at both developmental stage and tissue levels. Furthermore, RNA interference (RNAi)\u2010mediated knockdown of\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    resulted in increased mortality during the early larval stage. Injection of ds\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    at the beginning of the 4th instar caused a reduction in 20E titer, disrupted normal molting and pupation processes, and led to the emergence of larval\u2010pupal abnormalities. Notably,\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    expression was significantly down\u2010regulated on d 2 and 4 post\u2010injection, which coincided with a marked inhibition of\n                    <jats:italic>HvCHS1<\/jats:italic>\n                    and genes associated with 20E and bursicon signaling pathways. Additionally, silencing\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    induced qualitative changes in male testes and female ovaries, resulting in infertility and increased mortality. Ultimately, these findings suggest that\n                    <jats:italic>HvLmx1a<\/jats:italic>\n                    influences ovarian morphology and development through lipid metabolism. In conclusion, this study provides the first evidence of the diverse physiological roles of\n                    <jats:italic>Lmx1a<\/jats:italic>\n                    in\n                    <jats:italic>H. vigintioctopunctata<\/jats:italic>\n                    and highlight its potential as a target for RNAi\u2010based biological control strategies.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70033","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T14:38:34Z","timestamp":1743777514000},"page":"819-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Silencing of LIM homeodomain transcription factor 1 alpha (\n                    <i>Lmx1a<\/i>\n                    ) gene caused larval molting failure and adult reproductive deficiency in\n                    <i>Henosepilachna vigintioctopunctata<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Junna","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexin","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuguo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Illinois Urbana\u2010Champaign  Urbana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3508-6695","authenticated-orcid":false,"given":"Youjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Department of Plant Protection, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2299-5980","authenticated-orcid":false,"given":"Huipeng","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol Ministry of Education, South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.163394"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.10.037"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2005.00576.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1359"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-015-0543-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07779.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts565"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21833"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.20.120186.001051"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1883"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105428"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12899"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(99)01883-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.78"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2322927121"},{"key":"e_1_2_7_17_1","first-page":"1","volume-title":"Advances in Experimental Medicine and Biology","author":"Leyria J.","year":"2024"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-323"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.10.008"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-92"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01614"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12784"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrops.2024.100019"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612826666200523175409"},{"key":"e_1_2_7_26_1","first-page":"2874","article-title":"Study on management of damage caused by Henosepilachna vigintioctopunctata in brinjal","volume":"9","author":"Nithish A.","year":"2020","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5894"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01120"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.041244"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8822"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-012-0432-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000518323"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.07.007"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00093-07"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.20.4315"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2008.11.007"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.12.089"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809381115"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.07.006"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2048"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-011019-025224"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70033","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.70033","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:13:20Z","timestamp":1781331200000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.70033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,2]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/1744-7917.70033"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.70033","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,2]]},"assertion":[{"value":"2025-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}