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The AKH receptor is abundant in mosquito primary and secondary sexual organs; however, the exact role of the AKH receptor in mosquito reproductive processes and the specific mechanisms involved are unclear. Among different tissues of adult female mosquitoes (\n                    <jats:italic>Culex pipiens pallens<\/jats:italic>\n                    ), the ovaries and fat body showed high expression of the AKH receptor gene. Silencing this gene led to anti\u2010reproductive effects, including abnormal ovarian morphology, reduced follicle number, and a decreased egg\u2010laying count. Meanwhile, decreased yolk accumulation and iron deposition in the ovaries during the vitellogenesis phase were observed, accompanied by reduced expression levels of the vitellogenin\u2010A1 precursor gene and transferrin gene. Liquid chromatography\u2010mass spectrometry analysis revealed a decrease in L\u2010homoserine and serotonin levels following AKH receptor gene knockdown, and supplementation with the above 2 metabolites partly rescued the anti\u2010reproductive phenotype and increased the expression of the vitellogenin\u2010A1 precursor gene and transferrin gene in the AKH receptor gene knockdown mosquitoes. Consistent with the gene knockdown results, Relugolix, an inhibitor of this receptor, likewise affected egg production. Herein, we revealed evidence for the function and potential mechanism of the AKH receptor during female mosquito reproduction, possibly offering an alternative method to control mosquitoes.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70061","type":"journal-article","created":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T11:19:23Z","timestamp":1745752763000},"page":"1494-1512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Knockdown of the adipokinetic hormone receptor inhibits the reproduction of female\n                    <i>Culex pipiens pallens<\/i>\n                    (Diptera: Culicidae) by downregulating L\u2010homoserine and serotonin levels"],"prefix":"10.1111","volume":"33","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology, Key Laboratory for Pathogen Infection and Control of Jiangsu Province Nanjing Medical University 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