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The final metabolic step of hydrocarbon formation involves an oxidative decarbonylation carried out by cytochrome P450 from family 4, subfamily G (CYP4G).\n                    <jats:italic>Triatoma infestans<\/jats:italic>\n                    Klug (Hemiptera: Reduviidae: Triatominae) has two\n                    <jats:italic>CYP4G<\/jats:italic>\n                    genes, named\n                    <jats:italic>CYP4G106<\/jats:italic>\n                    and\n                    <jats:italic>CYP4G107<\/jats:italic>\n                    . In this study, we have silenced in\n                    <jats:italic>T. infestans<\/jats:italic>\n                    the expression of both genes by RNA interference in order to assess the relevance of hydrocarbons to survival, reproductive physiology, and insecticide susceptibility. Gene knockdown led to a significant decrease (\u2248 79%) in total cuticle hydrocarbons, especially normal alkanes. A reduced number of\n                    <jats:italic>CYP4G<\/jats:italic>\n                    \u2010silenced nymphs completed metamorphosis, and adult survival\u2014particularly among females\u2014was markedly diminished. Although mating behavior was not affected by silencing, the reproductive fitness was severely impaired as\n                    <jats:italic>CYP4G<\/jats:italic>\n                    \u2010silenced females laid about 77% fewer eggs per female than control females. Egg hatching was almost completely abolished by\n                    <jats:italic>CYP4G<\/jats:italic>\n                    \u2010silencing. Furthermore,\n                    <jats:italic>CYP4G<\/jats:italic>\n                    gene knockdown increased susceptibility to the insecticide deltamethrin, leading to a 40%\u201350% higher mortality compared to control insects. These findings highlight the essential role of\n                    <jats:italic>CYP4G<\/jats:italic>\n                    genes in cuticle function, survival, and reproductive fitness and suggest that disruption of hydrocarbon biosynthesis can enhance insecticide efficacy, making it a potential target for control of these insects.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70100","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T03:14:49Z","timestamp":1751858089000},"page":"2012-2024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The role of\n                    <i>CYP4G<\/i>\n                    s involved in hydrocarbon formation in the survival, reproduction, and pyrethroid resistance of\n                    <i>Triatoma infestans<\/i>\n                    , a major Chagas disease vector"],"prefix":"10.1111","volume":"33","author":[{"given":"Nicol\u00e1s Jes\u00fas Nazareth","family":"Ifr\u00e1n","sequence":"first","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas Instituto de Investigaciones Bioqu\u00edmicas de La Plata (CONICET\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Javier","family":"Mijailovsky","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas Instituto de Investigaciones Bioqu\u00edmicas de La Plata (CONICET\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianela","family":"Santana","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas Instituto de Investigaciones Bioqu\u00edmicas de La Plata (CONICET\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Rem\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad Operativa de Vectores y Ambiente, Centro Nacional de Diagnostico e Investigaci\u00f3n en Endemoepidemias (UnOVE\u2010CeNDIE) ANLIS Malbr\u00e1n, Ministerio de Salud de la Naci\u00f3n  Santa Mar\u00eda de Punilla Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Roberto","family":"Girotti","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas Instituto de Investigaciones Bioqu\u00edmicas de La Plata (CONICET\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2279-6209","authenticated-orcid":false,"given":"Gustavo Mario","family":"Calder\u00f3n\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas Instituto de Investigaciones Bioqu\u00edmicas de La Plata (CONICET\u2010UNLP)  La Plata 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